| 响应面法优化微生物除臭效果的研究 |
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| 引用本文： | 邵栓,党晓伟,李慧娟,常娟,王平,尹清强,高天增.响应面法优化微生物除臭效果的研究[J].中国畜牧兽医,2020,47(8):2684-2693. |
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| 作者姓名： | 邵栓 党晓伟 李慧娟 常娟 王平 尹清强 高天增 |
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| 作者单位： | 1. 河南农业大学牧医工程学院, 郑州 450002;2. 新疆玛纳斯县农业农村局, 昌吉 832200;3. 河南德邻生物制品有限公司, 新乡 453000;4. 河南省郑州种畜场, 郑州 450011;5. 河南广安生物科技股份有限公司, 郑州 450001 |
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| 基金项目： | 河南省重大科技专项（171100110500）；新乡市重大科技专项（ZD19005） |
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| 摘 要： | 为了减少畜禽粪便引发的恶臭气体污染，本研究选用具有除臭功能的贝莱斯芽孢杆菌、枯草芽孢杆菌、产朊假丝酵母和干酪乳杆菌4种微生物菌种作为Box-Behnke设计的4个因素，将4种菌种经活化后接入粪便中，以对应活菌数1×104、1×105和1×106 CFU/g作为Box-Behnke设计的3个编码水平。利用响应面设计构建得到29种复合微生物菌株组合，以其对猪粪中吲哚、氨和硫化氢的去除率作为参考指标，优化得到了4株除臭菌种的最优添加比例。结果表明，当菌液浓度为1×108 CFU/mL的贝莱斯芽孢杆菌、枯草芽孢杆菌、产朊假丝酵母和干酪乳杆菌分别以0.47%、0.05%、0.01%和1.00%的比例加入猪粪便中，堆积发酵7 d后对猪粪便中的吲哚、氨和硫化氢的去除率分别为73.59%、63.60%和70.29%。由此可见，经响应面设计优化后的除臭微生物菌种组合具有良好的除臭效果，对控制畜禽粪便恶臭污染具有较高的应用价值。
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| 关 键 词： | 粪便 吲哚 氨 硫化氢 微生物发酵 除臭 |
| 收稿时间： | 2019-12-09 |
Study on Optimizing the Effectiveness of Microbial Deodorization by Response Surface Methodology |
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| SHAO Shuan,DANG Xiaowei,LI Huijuan,CHANG Juan,WANG Ping,YIN Qingqiang,GAO Tianzeng.Study on Optimizing the Effectiveness of Microbial Deodorization by Response Surface Methodology[J].China Animal Husbandry & Veterinary Medicine,2020,47(8):2684-2693. |
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| Authors: | SHAO Shuan DANG Xiaowei LI Huijuan CHANG Juan WANG Ping YIN Qingqiang GAO Tianzeng |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Zhengzhou 450002, China;2. Manasi Bureau of Agriculture and Rural Affairs, Changji 832200, China;3. Henan Delin Biological Product Co., Ltd., Xinxiang 453000, China;4. Zhengzhou Breeding Farm of Henan Province, Zhengzhou 450011, China;5. Henan Guangan Biotechnology Co., Ltd., Zhengzhou 450001, China |
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| Abstract: | In order to reduce the odor pollution caused by livestock and poultry manure,four species of microbes with deodorization function such as Bacillus velezensis, Bacillus subtilis,Candida utilis and Lactobacillus casei were selected as 4 factors of experimental design in this study.The corresponding viable counts used in pig manure were 1×104,1×105,and 1×106 CFU/g as the three coding levels of the response surface design.In order to obtain an ideal microbial inoculation proportion for fecal deodorization,4 factors and 3 levels were used to construct 29 combinations of microorganisms in Box-Behnke design for removing indole,NH3 and H2S in feces.The results showed that the optimal ratio of Bacillus velezensis,Bacillus subtilis,Candida utilis and Lactobacillus casei obtained by response surface optimization were 0.47%,0.05%,0.01% and 1.00% at the concentration of 1×108 CFU/mL,respectively.After 7 d microbial fermentation,the removal rates of indole,NH3 and H2S in pig feces were 73.59%,63.60% and 70.29%,respectively.It could be concluded that an ideal compound microbial preparation for pig fecal innocent treatment had been obtained in this study,which provided the high application value to control the odor pollution caused by livestock and poultry manure. |
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| Keywords: | manure indole ammonia hydrogen sulfide microbial fermentation deodorization |
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