| 番茄叶夹角基因的精细定位 |
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| 引用本文： | 刘星雨,国艳梅,苏晓梅,黄泽军,王孝宣,杜永臣,高建昌.番茄叶夹角基因的精细定位[J].中国蔬菜,2020,1(1):31-37. |
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| 作者姓名： | 刘星雨 国艳梅 苏晓梅 黄泽军 王孝宣 杜永臣 高建昌 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，北京 100081 |
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| 基金项目： | 国家自然科学基金项目（31471874），“十三五”国家重点研发计划项目（2016YFD0101703），农业农村部园艺作物生物学和种质创制重点实验室项目，中央级公益性科研院所基本科研业务费专项（Y2017PT52） |
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| 摘 要： | 叶夹角作为株型的重要组成部分,是影响作物光能利用率和产量的重要因素。以叶片上举的161-817为父本,叶片平展的Heinz1706为母本构建F2∶3群体,对番茄叶夹角基因进行精细定位。遗传分析结果显示,叶上举对叶平展表现为部分显性。混池分析结合QTL作图结果显示,番茄10号染色体上存在2个主效QTL(LA1-1和LA1-2)与番茄叶夹角表型有关。其中,LA1-1位于分子标记A-10和A-5之间,遗传贡献率为18.7%,物理距离为1.58 Mb,该区域编码210个基因;LA1-2位于分子标记B-3和B-9之间,遗传贡献率为22.5%,物理距离为378 kb,该区域编码32个基因。
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| 关 键 词： | 番茄 株型 叶夹角 精细定位 |
Fine Mapping of Tomato Leaf Angle Genes |
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| LIU Xing-yu,GUO Yan-mei,SU Xiao-mei,HUANG Ze-jun,WANG Xiao-xuan,DU Yong-chen,GAO Jian-chang.Fine Mapping of Tomato Leaf Angle Genes[J].China Vegetables,2020,1(1):31-37. |
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| Authors: | LIU Xing-yu GUO Yan-mei SU Xiao-mei HUANG Ze-jun WANG Xiao-xuan DU Yong-chen GAO Jian-chang |
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| Institution: | Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing100081，China |
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| Abstract: | Leaf angle is an important part of plant type，which affecting crop photosynthetic efficiency and yield．Taking ‘Heinz1706’with open and flat leaves as female parent and‘161-817’with upthrow leaves as male parent，this study constructed F2：3 populations to conduct fine mapping of tomato leaf angle gene．Results of genetic analysis revealed that the leaf upthrow to open and flat leaf was shown partially dominant．The results of combining BSA method with QTL mapping indicated that there were 2 major QTL （LA1-1 and LA1-2）existed on chromosome of tomato No.10 and were connected with tomato leaf angle phenotype．Among them，LA1-1 was located between molecular markers A-10 and A-5 with 18.7% genetic contribution rate and 1.58 Mb physical distance．There were 210 genes encoded in this region．LA1-2 was located between molecular markers B-3 and B-9．The genetic contribution rates was 22.5% and physical distance was 378 kb．There were 32 genes encoded in this region． |
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| Keywords: | Tomato Plant type Leaf angle Exquisite location |
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