| 辣蓼黄酮正丁醇部分体外抗猪繁殖与呼吸综合征病毒的研究 |
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| 引用本文： | 周淑棉,曹迷霞,胡庭俊.辣蓼黄酮正丁醇部分体外抗猪繁殖与呼吸综合征病毒的研究[J].中国畜牧兽医,2020,47(2):605-611. |
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| 作者姓名： | 周淑棉 曹迷霞 胡庭俊 |
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| 作者单位： | 广西大学动物科学技术学院, 南宁 530005 |
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| 基金项目： | 国家自然科学基金（31560708） |
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| 摘 要： | 为明确辣蓼黄酮正丁醇部分（n-butanol part of flavonoids from Polygonum hydropiper L.，FNB）体外抗猪繁殖与呼吸综合征病毒（PPRSV）的效果。本研究以Marc-145细胞和PPRSV弱毒疫苗毒株（TJM-F92）为对象，通过CCK-8法检测FNB对细胞的毒性作用，并检测先给药后接毒、先接毒后给药、药物与病毒同时作用这3种方式处理细胞后药物对病毒的抑制率。结果发现，FNB对细胞的最大安全浓度为500 μg/mL，因此，选择25~500 μg/mL浓度范围的FNB进行后续试验。各浓度的FNB处理病毒后，能不同程度的抑制PRRSV在细胞上的增殖，并呈现一定的剂量效应关系，药物的浓度越高，抗病毒效果越好。其中，先接毒后给药、药物与病毒同时作用这两种方式抗PRRSV效果显著，在25~500 μg/mL浓度范围内细胞存活率分别为21.55%~65.23%和24.85%~73.60%。而先给药后接毒，不能有效降低病毒的感染力，在药物最高剂量（500 μg/mL）时细胞存活率仅为7.00%，抗病毒效果不明显。FNB预先作用于Marc-145细胞虽未降低PRRSV感染细胞的能力，即药物对于PRRSV预防作用效果不理想，但是FNB对病毒感染细胞后呈现一定的作用，药物能够通过抑制病毒的合成、释放及直接杀灭病毒，进而能够有效抑制PRRSV在细胞上的增殖。本试验结果不仅为FNB在临床上治疗猪繁殖与呼吸综合征（PRRS）提供参考依据，而且可以为辣蓼的深度开发和利用提供理论依据。
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| 关 键 词： | 辣蓼黄酮正丁醇部分(FNB) 猪繁殖与呼吸综合征病毒(PRRSV) 抗病毒 抑制效果 细胞存活率 |
| 收稿时间： | 2019-08-29 |
A Study on Effect of Anti-PRRSV of n-butanol Part of Flavonoids from Polygonum hydropiper L. in vitro |
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| ZHOU Shumian,CAO Mixia,HU Tingjun.A Study on Effect of Anti-PRRSV of n-butanol Part of Flavonoids from Polygonum hydropiper L. in vitro[J].China Animal Husbandry & Veterinary Medicine,2020,47(2):605-611. |
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| Authors: | ZHOU Shumian CAO Mixia HU Tingjun |
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| Institution: | College of Animal Science and Technology, Guangxi University, Nanning 530005, China |
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| Abstract: | To investigate the effect of n-butanol part of flavonoids from Polygonum hydropiper L.(FNB) against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro,in this study,Marc-145 cells and PRRSV (TJM-F92) were used as the objectives,the toxicity of FNB on cells was detected by CCK-8 method.In addition,the inhibition rate of the FNB on PRRSV after the cells were treated by three methods (drug administration before PRRSV inoculation,drug administration post PRRSV inoculation,drug administration and PRRSV inoculation simultaneously).The results showed that the maximum safe concentration of FNB was 500 μg/mL.Therefore,FNB in the concentration range of 25-500 μg/mL was selected for subsequent experiments.Various concentrations of FNB could inhibit the proliferation of PRRSV on Marc-145 cells to varying degrees and exhibited a dose-dependent relation,which meant the higher the concentration of the drug,the better the antiviral effect.Among them,the last two treatment methods had significant anti-PRRSV effects,the cell viability was 21.55%-65.23% and 24.85%-73.60% respectively in the concentration range of 25-500 μg/mL.However,the infection of PRRSV after first administration couldn’t effectively reduce the infectivity of the PRRSV and the cell survival rate was only 7.00% at the highest dose of 500 μg/mL,so the antiviral effect was not obvious.Although the pretreatment of FNB on Marc-145 cells did not reduce the ability of PRRSV infection,the preventive effect of FNB on PRRSV cells was not satisfactory.However,the effect of FNB on the clinical treatment of PRRSV was considerable.FNB could inhibit the synthesis and release of PRRSV and directly kill of PRRSV,and then effectively inhibit the expression of PRRSV in Marc-145 cells.The results of this study not only provide a reference for the clinical treatment of PRRSV,but also offer theoretical basis for the further development and utilization of Polygonum hydropiper L.. |
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| Keywords: | n-butanol part of flavonoids from Polygonum hydropiper L (FNB) PRRSV antiviral inhibitory effect cell viability |
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