| 鸡爪槭（Acer palmatum Thunb.）品种‘赤枫’组培再生体系的建立 |
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| 引用本文： | 马建华,朱晓菲,何程相,丁龙梅,向明剑,黄良伟,姜良珍,谢松林.鸡爪槭（Acer palmatum Thunb.）品种‘赤枫’组培再生体系的建立[J].中国农业科技导报,2020,22(1):171-178. |
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| 作者姓名： | 马建华 朱晓菲 何程相 丁龙梅 向明剑 黄良伟 姜良珍 谢松林 |
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| 作者单位： | 1.四川七彩林科股份有限公司研究院, 四川省名特优新彩色苗木繁育工程实验室, 四川 巴中 635600;2.成都大学药学与生物工程学院, 成都 610106 |
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| 基金项目： | 2018年四川省知识产权专项。 |
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| 摘 要： | 鸡爪槭品种‘赤枫’是稀有的生长季观叶，冬季观枝的园林彩化树种。为了建立合理的组培再生体系，在短时期内获得大量种苗，以‘赤枫’带芽茎段为外植体，对其灭菌方式及启动、增殖、生根培养基进行了筛选，并对所获组培苗移栽技术进行了探讨。结果显示：全年中6月份为最佳外植体采集时间，外植体经50%多菌灵400倍液浸1 h后，以75％乙醇30 s+0.1％升汞10 min浸泡处理的灭菌效果最佳。再生体系各阶段最适培养基为：①启动培养：MS+0.5 mg·L-1 IAA；②增殖培养：MS+2.0 mg·L-1 6-BA+0.5 mg·L-1 IAA；③生根培养：1/2MS+0.3 mg·L-1 NAA。组培苗闭瓶炼苗6 d，开瓶炼苗3 d后，移栽到草炭∶蛭石∶珍珠岩=1∶1∶1的基质中，炼苗成活率可达90%。
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| 关 键 词： | 鸡爪槭 &lsquo 赤枫&rsquo 组培 再生体系 |
| 收稿时间： | 2018-11-13 |
Regeneration of Acer Palmatum cv. ‘Sango kaku’ by Tissue Culture |
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| MA Jianhua,ZHU Xiaofei,HE Chengxiang,DING Longmei,XIANG Mingjian,HUANG Liangwei,JIANG Liangzhen,XIE Songlin.Regeneration of Acer Palmatum cv. ‘Sango kaku’ by Tissue Culture[J].Journal of Agricultural Science and Technology,2020,22(1):171-178. |
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| Authors: | MA Jianhua ZHU Xiaofei HE Chengxiang DING Longmei XIANG Mingjian HUANG Liangwei JIANG Liangzhen XIE Songlin |
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| Institution: | 1.Sichuan Provincial Engineering Laboratory on Precious Colorful Plants, Sichuan Colorful Forest Development Co., Ltd. R & D Department, Sichuan Bazhong 635600, China; 2.College of Pharmacy and Biological Engineering, Chengdu University, Chengdu 610106, China |
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| Abstract: | Acer Palmatum cv. ‘Sango kaku’ is a precious ornamental tree with colorful foliage in growing season and red stems in winter. In order to establish the tissue culture regeneration system, taking the bud stem segment of ‘Sango kaku’ as explant, the sterilization and medium for induction, proliferation and rooting were selected, and the transplanting techniques were established in vitro plants. The results showed that, June was the best timimg of the whole year for explant collection. The explant samples were sterilized with 75% ethanol for 30 s plus 0.1% mercury for 10 min after immersion in 400× Carbendazim, which was the best sterilization way. The optimum medium for each stage of the regeneration system was: ① induction culture: MS+0.5 mg·L-1 IAA; ② proliferation culture: MS+2.0 mg·L-1 6-BA+0.5 mg·L-1 IAA; ③ rooting culture: 1/2 MS+0.2 mg·L-1 NAA. The tissue culture seedlings were kept in closed bottle for 6 d, in opening bottle for 3 d, and transplanted to the matrix with charcoal∶vermiculite∶perlite = 1∶1∶1. The survival rate of the transplanted tissue culture plants could reach 90%. |
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| Keywords: | Acer palmatum ‘Sango kaku’ tissue culture regeneration system |
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