| 基于叶绿素荧光参数的滴灌棉花氮素营养估测模型 |
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| 引用本文： | 李冬梅,吕新,罗宏海,祁立敏,丁怡人,马金鑫,哈发都曼,马露露,张泽.基于叶绿素荧光参数的滴灌棉花氮素营养估测模型[J].棉花学报,2020,32(1):63-76. |
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| 作者姓名： | 李冬梅 吕新 罗宏海 祁立敏 丁怡人 马金鑫 哈发都曼 马露露 张泽 |
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| 作者单位： | 石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室;新疆生产建设兵团第八师石河子总场 |
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| 基金项目： | 国家重点研发计划子课题(2017YFD0700503-3);中国博士后科学基金(2017M623282);石河子大学创新发展专项(CXFZ201903)。 |
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| 摘 要： | 【目的】建立基于叶绿素荧光参数的棉花叶片氮素营养监测模型,为棉花高效施氮及植株生长状况的无损监测提供方法。【方法】在水肥一体化滴灌条件下,以新陆早58号为试验材料,设置4个施氮水平,测定了棉花生长关键时期顶2(从顶部数起)至顶5叶的叶绿素荧光参数和氮素含量,分析了棉花叶片氮素含量与荧光参数的关系。【结果】(1)棉花出苗70 d后,叶片氮素含量以及光系统Ⅱ(PSⅡ)潜在光化学活性(Fv/F0)、PSⅡ潜在最大光化学效率(Fv/Fm)、PSⅡ实际光化学效率(ΦPSⅡ)、PSⅡ最大光化学效率(Fv’/Fm’)均随着棉花生长呈逐渐下降趋势。(2)不同氮素处理下,各叶片氮素含量以及叶绿素荧光参数Fv/F0、Fv/Fm、ΦPSⅡ和Fv’/Fm’均随施氮量的增加而上升,其中N2(240 kg·hm-2)处理下各荧光参数值最大。(3)叶片氮含量与Fv、Fv/F0、Fv/Fm、ΦPSⅡ都呈现极显著正相关关系,与荧光参数ΦPSⅡ、Fv’/Fm’间呈现较好的指数函数关系,与荧光参数Fv、Fv/Fm、Fv/F0呈现良好的线性函数关系。顶5叶可变荧光(Fv)与叶片氮素含量建立的关系模型(y=0.0022x+1.6243)的模拟效果最好,决定系数达到0.928,相关系数达到0.963,呈极显著正相关。【结论】适量施氮(240 kg·hm-2)能够提高PSⅡ的活性及PSⅡ反应中心开放部分的比例,进而改善棉花叶片光合能力。顶5叶氮素含量与叶绿素荧光参数Fv构建的模型精确度和拟合效果较其他荧光参数好,因此可以选用荧光参数Fv来监测棉花叶片氮素含量,进而监测植株的氮素营养状况。
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| 关 键 词： | 施氮水平 棉花 叶绿素荧光 氮素 估测 |
A Monitoring Model of Nitrogen Nutrition of Drip Irrigation Cotton Based on Chlorophyll Fluorescence Parameters |
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| Li Dongmei,Lü Xin,Luo Honghai,Qi Limin,Ding Yiren,Ma Jinxin,Ha Faduman,Ma Lulu,Zhang Ze.A Monitoring Model of Nitrogen Nutrition of Drip Irrigation Cotton Based on Chlorophyll Fluorescence Parameters[J].Cotton Science,2020,32(1):63-76. |
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| Authors: | Li Dongmei Lü Xin Luo Honghai Qi Limin Ding Yiren Ma Jinxin Ha Faduman Ma Lulu Zhang Ze |
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| Institution: | 1.Agricultural College of Shihezi University/ Key Laboratory of Oasis Ecological Agriculture of Xinjiang Production and Construction Corps, Shihezi, Xinjiang 832000, China; 2.The General Farm of Eighth Division in Xinjiang production and construction Corps. Shihezi, Xinjiang 832000, China |
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| Abstract: | Objective] A monitoring model of nitrogen nutrition of cotton leaves based on chlorophyll fluorescence parameters was established for non-destructive monitoring of high efficiency nitrogen application and plant growth of cotton. Method] A field study was conducted by using Xinluzao 58 as experimental material with four input levels of nitrogen, which were applied under the condition of mulched drip irrigation. Chlorophyll fluorescence parameters and nitrogen content of leaves in different positions of main stem were measured during the yield formation period of cotton, and the relationship between nitrogen content and fluorescence parameters of cotton leaves was analyzed. Results](1) After 70 days of cotton emergence, the potential photochemical activity(Fv/F0) of photosystem II(PSⅡ), the potential maximum photochemical efficiency of PSII (Fv/Fm), the actual photochemical efficiency of PSII (ΦPSII) and the maximal photochemical efficiency (Fv'/Fm') of the top 2 to top 5 leaves showed a gradual downward trend with the growth of cotton. (2) The nitrogen content and fluorescence parameters (Fv/F0, Fv/Fm, ΦPSII and Fv'/Fm') of top 2 to top 5 leaves showed an upward trend with the increase of nitrogen application rate, the N2 (240 kg·hm－2) treatment had the highest values of chlorophyll fluorescence parameters. (3) The nitrogen content in leaves showed significant and positive correlation with Fv, Fv/F0, Fv/Fm and ΦPSII, and a good exponential function relationship with fluorescence parameters ΦPSII and Fv'/Fm', and a good linear function relationship with fluorescence parameters Fv, Fv/Fm and Fv/F0. The model between Fv and nitrogen content of top5 leaf(y＝0.0022x＋1.6243) had the best simulation effect, showing an extremely significant correlation, with the determination coefficient reaching 0.928 and the correlation coefficient reaching 0.963. Conclusion] Proper applying of nitrogen (240 kg·hm－2) could increase the activity of PSII and the proportion of the open part of PSII reaction center and improve the photosynthetic capacity of cotton leaves. Nitrogen content in top 5 leaf was significantly correlated with chlorophyll fluorescence parameter Fv, which was therefore can be used to monitor nitrogen content in cotton leaves and further monitor nitrogen nutritional status of cotton plants. |
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| Keywords: | nitrogen level cotton chlorophyll fluorescence nitrogen estimation |
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