| 日粮添加罗伊氏乳杆菌LR1对猪常见病毒疫苗免疫效果的影响 |
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| 引用本文： | 冯军森,熊云霞,吴绮雯,王丽,蒋宗勇,易宏波.日粮添加罗伊氏乳杆菌LR1对猪常见病毒疫苗免疫效果的影响[J].中国畜牧兽医,2020,47(3):884-891. |
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| 作者姓名： | 冯军森 熊云霞 吴绮雯 王丽 蒋宗勇 易宏波 |
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| 作者单位： | 1. 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 国家畜禽育种重点实验室, 广东省动物育种与营养公共实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;2. 华南农业大学兽医学院, 广州 510642 |
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| 基金项目： | 国家重点研发计划项目（2018YFD0501101）；广东省自然科学基金项目（2018A030310191）；广东省农业科学院农业科技推广服务网络建设项目（2018分院2-6） |
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| 摘 要： | 试验利用前期筛选的猪源罗伊氏乳杆菌LR1全程替代饲用抗生素，旨在研究其对猪常见病毒疫苗免疫效果的影响。试验选取144头21日龄杜洛克×长白×大白断奶仔猪，随机分为3组，每组8个重复，每个重复6头猪。对照组（CON）饲喂基础日粮，抗生素组（OA）饲喂基础日粮+100 mg/kg喹乙醇+75 mg/kg金霉素（抗生素在第120天停用），益生菌组（LR1）饲喂基础日粮+5×1010 CFU/kg罗伊氏乳杆菌LR1，试验周期166 d。试验所有猪根据统一免疫程序进行疫苗免疫，并于试验开始第14、42、120、127、134、166天检测猪血清中猪繁殖与呼吸综合征病毒（PRRSV）、猪瘟病毒（HCV）、猪口蹄疫病毒（FMDV）、伪狂犬病毒（PRV）、细小病毒（PPV）抗体水平以及血清中IgG含量。结果表明：①与对照组和抗生素组相比，益生菌组第14天血清中口蹄疫抗体水平显著提高（P<0.05）；②与对照组相比，益生菌组和抗生素组第120天血清中的细小病毒抗体水平均显著提高（P<0.05）；③益生菌组第166天血清中的猪繁殖与呼吸综合征病毒抗体水平显著高于对照组（P<0.05），且益生菌组第166天血清中的猪瘟抗体水平显著高于对照组和抗生素组（P<0.05）。综上所述，日粮添加罗伊氏乳杆菌LR1在一定程度上能够提高猪常见病毒疫苗免疫后的抗体水平，提示其不仅可以作为饲用抗生素的潜在替代品，同时在预防和治疗猪病毒性疾病方面具有应用前景。
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| 关 键 词： | 罗伊氏乳杆菌LR1 抗生素 猪 抗体水平 IgG |
| 收稿时间： | 2019-10-18 |
The Effect of Lactobacillus reuteri LR1 on the Immune Response of Swine Common Virus Vaccine |
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| FENG Junsen,XIONG Yunxia,WU Qiwen,WANG Li,JIANG Zongyong,YI Hongbo.The Effect of Lactobacillus reuteri LR1 on the Immune Response of Swine Common Virus Vaccine[J].China Animal Husbandry & Veterinary Medicine,2020,47(3):884-891. |
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| Authors: | FENG Junsen XIONG Yunxia WU Qiwen WANG Li JIANG Zongyong YI Hongbo |
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| Institution: | 1. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agricultur and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | In order to study the effect of Lactobacillus reuteri LR1 isolated earlier on immunological effect of swine common viral vaccines,the whole process of Lactobacillus reuteri LR1 was used instead of feed antibiotics.A total of 144 weaned pigs (21 days old,Duroc×Landrace×Large White) were selected and randomly divided into 3 groups with 8 replicates in each group and 6 pigs in each replicate.The control group (CON) was fed basic diet,and the antibiotic group (OA) was fed basic diet +100 mg/kg olaquindox +75 mg/kg aureomycin,and the probiotics group (LR1) was fed basic diet +5×1010 CFU/kg Lactobacillus reuteri LR1.The use of antibiotics was stopped at 120 d,and the experimental period was 166 d.All pigs were immunized according to the unified immunization procedure.The antibody levels of porcine reproductive and respiratory syndrome virus (PRRSV),Hog cholera virus (HCV),foot-and-mouth disease virus (FMDV),pseudorabies virus (PRV),porcine parvovirus (PPV) and the level of IgG were detected in the serum of pigs at the 14th,42nd,120th,127th,134th and 166th days after the test.The results showed that,compared with the control group and the antibiotic group,the antibody level of FMDV in the serum of probiotics group on the 14th day was significantly increased (P<0.05).Compared with the control group,the antibody level of PPV in the serum of probiotics group and antibiotic group on the 120th day were significantly increased (P<0.05).The antibody level of PRRSV in the serum of the probiotics group on the 166th day was significantly higher than that of the control group (P<0.05).The antibody level of HCV in the serum of the probiotics group on the 166th day was significantly higher than those of the control group and the antibiotic group (P<0.05).In conclusion,dietary Lactobacillus reuteri LR1 supplementation could improve the antibody levels of common viruses in commercial pigs to a certain extent.These data suggested that Lactobacillus reuteri LR1 can be used to develop new substitutes for antibiotic,and has a great potential in the prevention and treatment of diseases during replacing dietary antibiotics. |
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| Keywords: | Lactobacillus reuteri LR1 antibiotics pigs antibody level IgG |
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