| Pb胁迫下内生真菌侵染对德兰臭草生长及生理的影响 |
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| 引用本文： | 李柯,施宠,何飞焱,李昊宇.Pb胁迫下内生真菌侵染对德兰臭草生长及生理的影响[J].草业学报,2020,29(3):112-120. |
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| 作者姓名： | 李柯 施宠 何飞焱 李昊宇 |
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| 作者单位： | 新疆农业大学草业与环境科学学院,新疆 乌鲁木齐 830052 |
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| 基金项目： | 新疆维吾尔自治区自然科学基金面上项目(2016D01A041)资助 |
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| 摘 要： | 采用盆栽试验,对带菌(E+)和不带菌(E-)的德兰臭草进行不同浓度的铅(Pb)胁迫处理,探究Pb胁迫下内生真菌侵染对德兰臭草生长及生理的影响。结果表明,随着Pb浓度的升高,德兰臭草的株高呈现先增加后减少的趋势,当胁迫浓度达到1500 mg·L-1时,德兰臭草的株高受到了显著的抑制,且根鲜重的下降幅度最大,显著低于对照82.5%(P<0.05);德兰臭草叶片中MDA含量显著增加(P<0.05),在胁迫浓度为2000 mg·L-1时,E+植株MDA含量显著高于对照87.3%(P<0.05);德兰臭草体内的脯氨酸含量也呈现先增加后减少的趋势,当胁迫浓度达到500 mg·L-1时,E+植株脯氨酸含量显著高于对照35.2%(P<0.05)。与不带菌植株相比,E+植株地上和地下的生物量均高于E-植株;E+德兰臭草的MDA显著低于E-(P<0.05),在Pb浓度为2000 mg·L-1时,E+植株的MDA含量显著低于E-25.0%(P<0.05);E+植株的脯氨酸含量在Pb浓度为1500 mg·L-1时显著高于E-植株84.1%(P<0.05)。总的来说,感染内生真菌的德兰臭草减轻了Pb对宿主的毒害,增强其对重金属Pb胁迫的生理生化适应能力。
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| 关 键 词： | 德兰臭草 内生真菌 铅胁迫 生理特性 |
| 收稿时间： | 2019-06-13 |
Effects of endophyte infection on growth and physiological characteristics of Melica transsilvanica under Pb stress |
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| LI Ke,SHI Chong,HE Fei-yan,LI Hao-yu.Effects of endophyte infection on growth and physiological characteristics of Melica transsilvanica under Pb stress[J].Acta Prataculturae Sinica,2020,29(3):112-120. |
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| Authors: | LI Ke SHI Chong HE Fei-yan LI Hao-yu |
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| Institution: | College of Pratacultural and Environmental Sciences, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | The growth and physiological characteristics of Epichloё endophyte infected (E+) and endophyte free (E-) Melica transsilvanica were observed under different Pb concentrations (0, 200, 500, 1000, 1500, 2000 mg·L-1) in a pot experiment. It was found that with increase in Pb concentration, plant height initially increased and then decreased. At the Pb concentration of 500 mg·L-1, the proline content of E+ plants was 35.2% higher (P<0.05) than in the Controls. At Pb concentrations of 1500 mg·L-1 and above, plant height was significantly inhibited. The root fresh weight exhibited the largest decrease, being 82.5% lower than the control (P<0.05); meanwhile, the MDA concentration in leaves increased significantly. When the Pb concentration was 2000 mg·L-1, the MDA content of E+ plants were significantly higher than the Control (P<0.05), and the content of proline in the plant increased initially, and then decreased. Compared with M. transsilvanica plants without Epichloё endophytic fungi, the aboveground and underground biomass of E+ plants were both higher than in E- plants; the proline content of E+ plants was significantly higher than in the E- plants at the 1500 mg·L-1 Pb concentration (P<0.05); and the MDA content of E+ plants was 25% lower than in E- plants (P<0.05), especially at the 2000 mg·L-1 Pb concentration. In conclusion, the growth and physiological adaptability to Pb stress of M. transsilvanica infected with endophyte fungi was enhanced. |
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| Keywords: | Melica transsilvanica endophyte Pb stress physiological characteristics |
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