| 吉陶单极虫地理分布新记录及不同地理株系间的比较 |
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| 引用本文： | 陈鸿真,赵元莙,杨承忠.吉陶单极虫地理分布新记录及不同地理株系间的比较[J].水产学报,2021,45(2):265-273. |
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| 作者姓名： | 陈鸿真 赵元莙 杨承忠 |
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| 作者单位： | 重庆师范大学,重庆师范大学,重庆师范大学 |
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| 基金项目： | 国家自然科学基金(No. 31970409；No. 31672280)；重庆市自然科学基金(No. cstc2018jcyjAX0738)；重庆市留学人员回国创业创新支持计划(No. cx2018108)；重庆市教委科学技术研究项目(No. KJQN201900505) |
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| 摘 要： | 目的]为获知吉陶单极虫不同地理株系间的遗传变异及系统发育关系；方法]对吉陶单极虫中国重庆、中国湖北、中国四川、日本盐田、韩国全北、韩国首尔等地理株系进行形态学的比较及基于18S rDNA序列的变异位点、相似度、遗传距离、基因型的比较和系统发育分析；结果]吉陶单极虫各株系孢子形态特征基本一致；18S rDNA序列相似度为99.5%~100.0%，变异位点数为0~6个，遗传距离为0.000~0.004；系统发育分析显示，大部分有鞘膜的单极虫聚为一支，无鞘膜的单极虫聚为另一枝；无鞘膜单极虫依据寄生部位的不同形成了特殊的鳍寄生支系和鳃寄生支系；结论]吉陶单极虫鞘膜不宜作为该物种鉴定的主要依据，该物种的鉴定仍应以孢子本身的形态及量度为主；虽然单极虫鞘膜的形状和大小并不稳定，但物种自身有无鞘膜可能与其系统发育相关，且单极虫有鞘膜支系与无鞘膜支系可能有着不同的进化模式；吉陶单极虫并未形成与寄生部位相关的特殊分化也未形成地理种群特有的单系。这种遗传格局产生的原因可能是各地理分布区吉陶单极虫来源于一个共同的祖先株系且各地理株系之间隔离的时间较短或存在较频繁的基因交流，尚未形成较大的种群分化。
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| 关 键 词： | 吉陶单极虫 18S rDNA 株系比较 地理新记录 |
| 收稿时间： | 2019/9/16 0:00:00 |
| 修稿时间： | 2020/8/11 0:00:00 |
New record of geographic distribution of Thelohanellus kitauei and its comparative study of different geographic strains |
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| CHEN Hongzhen,ZHAO Yuanjun,YANG Chengzhong.New record of geographic distribution of Thelohanellus kitauei and its comparative study of different geographic strains[J].Journal of Fisheries of China,2021,45(2):265-273. |
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| Authors: | CHEN Hongzhen ZHAO Yuanjun YANG Chengzhong |
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| Institution: | Chongqing Normal University,,Chongqing Normal University |
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| Abstract: | Purposes] To clarify the genetic variation and phylogenetic relationships among different geographic strains of Thelohanellus kitauei. Methods] Comparative analyses were conducted on strains of T. kitauei from Chongqing of China, Hubei of China, Shioda of Japan, Jeonbuk of Korea and Seoul of Korea based on their morphology. The comparison and analyses were also conducted for their site variation, similarity, genetic distance, genotype and phylogeny based on 18S rDNA sequences. Findings] The morphological characteristics of strains of T. kitauei from different localities were generally consistent. Among different strains, the similarities were 99.5%~100.0%, variation sites were 0~6, genetic distances were 0.000~0.004, based on 18S rDNA sequences. The phylogenetic analysis showed that thelohanellids with membrane sheath clustered as a clade and the remaining thelohanellids without membrane sheath clustered as another clade. The clade without membrane sheath divided into fin-infection group and gill-infection group. Conclusions] The membrane sheath should not use as a valid feature for species identification of T. kitauei whose identification should still be according to its morphology and morphometry of the spore excluding membrane sheath. Though the shape and size of membrane sheath was unstable, its present and absence on thelohanellid might associate with phylogeny of Thelohanellus. And the evolution pattern for clade possessing membrane sheath might be different from the clade without membrane sheath. The strains of T. kitauei did not form special divergences in different parasitic sites and also did not form monophylies in different localities. The reason for this genetic situation might be: the isolation time was not long enough or the high frequence of gene flow among different strains which lead a limited population divergence. |
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| Keywords: | Thelohanellus kitauei new geographic record 18S rDNA comparison of strains |
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