| 龙眼‘蜀冠’ב大乌圆’杂交后代果实品质性状多样性分析及综合评价 |
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| 引用本文： | 薛 鑫,石胜友,侯世奎.龙眼‘蜀冠’ב大乌圆’杂交后代果实品质性状多样性分析及综合评价[J].园艺学报,2020,36(5):827-836. |
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| 作者姓名： | 薛 鑫 石胜友 侯世奎 |
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| 作者单位： | 1岭南师范学院生命科学与技术学院，广东湛江 524048；2中国热带农业科学院南亚热带作物研究所，农业部热带果树生物学重点实验室，广东湛江 524091；3海南大学园艺学院，海口 570100；4重庆市储美果树研究所，重庆 402260 |
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| 基金项目： | 国家自然科学基金项目（31572087）；广东省自然科学基金项目（2020A1515011365）；国家现代农业产业技术体系建设专项资金项目（CARS-32-02）；中国热带农业科学院基本科研业务费项目（1630062020003） |
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| 摘 要： | 以龙眼‘蜀冠’ב大乌圆’的F1代65个单株为材料，对其果实的27个品质性状进行了连续3年的观测，应用SPSS19.0软件对各性状进行变异系数、Shannon-Weinner多样性指数和主成分分析。结果表明：65个单株果实品质性状存在着极显著差异，13个数量性状（单果质量、果实纵径、果实横径、可溶性固形物、可食率、果皮厚度、果皮质量、果肉厚、种子质量、维生素C含量、蔗糖含量、葡萄糖含量和果糖含量）变异系数在6.95% ~ 41.64%之间；多样性指数在1.33 ~ 2.02之间；14个描述性状（成熟期、果皮颜色、果形、果肩、果顶、龟裂纹、疣状突起、果肉颜色、果肉质地、果肉透明度、果肉离核难易度、果汁、化渣程度和果肉风味）多样性指数在0.56 ~ 1.28之间；主成分分析发现前5个性状（单果质量、可食率、可溶性固形物、成熟期和果实风味）的累积贡献率为87.50%；建立了‘蜀冠’ב大乌圆’杂交后代果实品质性状综合评价的方法，利用该方法在65个杂交后代中筛选出了5个优异的单株（SD7、SD11、SD36、SD53和SD120），结果与实际观测结果高度一致。
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| 关 键 词： | 龙眼 杂交后代 果实品质性状 多样性 综合评价 |
Specific expression of hyperpolarization-activated cyclic nucleotide-ga-ted cation channel isoforms in rat superficial spinal dorsal horn |
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| HUANG Jiao-yan,CHENG Xiao-e,MA Long-xian,ZHANG Da-ying,JIANG Chang-yu,LIU Tao.Specific expression of hyperpolarization-activated cyclic nucleotide-ga-ted cation channel isoforms in rat superficial spinal dorsal horn[J].Acta Horticulturae Sinica,2020,36(5):827-836. |
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| Authors: | HUANG Jiao-yan CHENG Xiao-e MA Long-xian ZHANG Da-ying JIANG Chang-yu LIU Tao |
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| Institution: | 1.School of Life and Technology of Lingnan Normal University，Zhanjiang，Guangdong 524048，China；2Key Laboratory of Tropical Fruit Biology，Ministry of Agriculture，South Subtropical Crops Research Institute，Chinese Academy of Tropical Agricultural Sciences，Zhanjiang，Guangdong 524091，China；3College of Horticulture and Landscape Architecture，Hainan University，Haikou 570100，China；4Chumei Subtropical Fruit Trees Research Institute，Chongqing 402260，China |
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| Abstract: | AIMTo investigate the distinct expression and localization of the 4 subtypes of hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channels in rat superficial spinal dorsal horn (SDH). METHODSTransverse slices of L4~L5 segments were obtained from Sprague-Dawley (SD) rats (3~5-week-old) of either sex. Using the immunohistochemical technique and laser confocal imaging, the distribution of HCN1~4 in neurons, glia cells and subcellular structure of neuron in SDH was observed. RESULTSThe expression of 4 subtypes of HCN channel in rat SDH was distinct: HCN1 was mainly co-localized with neuronal marker neuronal nuclei (NeuN) and astrocyte marker glial fibrillary acidic protein (GFAP). HCN2 and HCN3 were largely co-localized with NeuN. HCN2 was primarily co-localized with peptidergic primary afferent nerve ending marker calcitonin gene-related peptide (CGRP). However, HCN4 was mainly co-localized with non-peptidergic primary afferent nerve ending marker isolectin B4 (IB4). HCN1 and HCN4 were mainly co-localized with dendrite marker microtubule-associated protein 2 (MAP2). HCN4 was primarily co-localized with axonal terminal of inhibitory interneuron marker vesicular γ-aminobutyric acid transporter (VGAT). CONCLUSION HCN1~4 are predominantly distributed in superficial spinal dorsal horn and exhibit a specific expression pattern in neurons, glia cells and subcellular structure of neurons. |
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| Keywords: | Hyperpolarization-activated cyclic nucleotide-gated cation channels Spinal dorsal horn Neurons |
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