| 四川省甘孜州牦牛梨形虫感染情况的调查 |
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| 引用本文： | 蓝岚,李友英,杨丹娇,潘瑶,陈冬明,贺安祥,汤承.四川省甘孜州牦牛梨形虫感染情况的调查[J].中国畜牧兽医,2020,47(5):1514-1522. |
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| 作者姓名： | 蓝岚 李友英 杨丹娇 潘瑶 陈冬明 贺安祥 汤承 |
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| 作者单位： | 1. 西南民族大学生命科学与技术学院, 成都 610041;2. 甘孜藏族自治州畜牧业科学研究所, 康定 626000;3. 四川省肉牛创新团队, 成都 610041 |
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| 基金项目： | 牛羊重要疫病诊断与检测新技术研究（2016YFD0500900） |
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| 摘 要： | 试验旨在调查甘孜藏族自治州（甘孜州）全部18个县（市）牦牛梨形虫的感染情况。2018年6月至11月采集甘孜州18个县（市）临床健康牦牛全血1 381份，采用靶向18S rRNA的巢式PCR进行梨形虫核酸检测，并挑选梨形虫核酸检测阳性样本PCR产物共计124份测序鉴定虫种。结果显示，1 381份牦牛样本中梨形虫核酸阳性样本438份，平均阳性率为31.72%，其中半农半牧业县1 093份牦牛样本中梨形虫核酸阳性样本385份，平均阳性率为35.22%，纯牧业县288份牦牛样本中梨形虫核酸阳性样本53份，平均阳性率为18.40%；124份牦牛梨形虫核酸阳性样本中鉴定出感染的虫种有中华泰勒虫76份、吕氏泰勒虫25份、东方泰勒虫3份、绵羊泰勒虫2份、双芽巴贝斯虫1份和水牛泰勒虫1份，其中中华泰勒虫感染率为61.29%，吕氏泰勒虫感染率为20.16%。本试验结果表明，甘孜州牦牛梨形虫感染率较高，不同地区之间存在差异，优势虫种为中华泰勒虫和吕氏泰勒虫，为甘孜州牦牛梨形虫病防控提供了重要参考。
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| 关 键 词： | 梨形虫 感染情况 牦牛 |
Investigation on the Infection of Piroplasma in Yak in Ganzi Prefecture of Sichuan Province |
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| LAN Lan,LI Youying,YANG Danjiao,PAN Yao,CHEN Dongming,HE Anxiang,TANG Cheng.Investigation on the Infection of Piroplasma in Yak in Ganzi Prefecture of Sichuan Province[J].China Animal Husbandry & Veterinary Medicine,2020,47(5):1514-1522. |
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| Authors: | LAN Lan LI Youying YANG Danjiao PAN Yao CHEN Dongming HE Anxiang TANG Cheng |
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| Institution: | 1. College of Life Science and Technology Southwest Minzu University, Chengdu 610041, China;2. Animal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding 626000, China;3. Sichuan Beef Cattle Innovation Team, Chengdu 610041, China |
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| Abstract: | The purpose of this study was to investigate the infection of Piroplasma in yak in all 18 counties (cities) of Ganzi Tibetan autonomous prefecture (Ganzi prefecture).From June to November 2018,a total of 1 381 whole blood samples of clinical healthy yak were collected in all 18 counties of Ganzi prefecture.A nested PCR targeting 18S rRNA was used to detect Piroplasma.Furthermore,a total of 124 positive PCR products were randomly selected to identify parasite species.The results showed that 438 of 1 381 yak blood samples were detected as Piroplasma positive with an average positive rate of 31.72%.Specifically,the 385 of 1 093 yak blood samples were detected as Piroplasma positive in semi-agricultural and semi-pastoral counties,the average positive rate was 35.22%.53 of 128 yak blood samples were detected as Piroplasma positive in pure pastoral counties,the average positive rate was 18.40%.In 124 positive PCR products,the species infecting yak were Theileria sinensis (76),Theileria luwenshuni (25),Theileria orientalis (3),Theileria ovis (2),Babesia bigemina (1) and Theileria buffeli (1),respectively.The infection rate of Theileria sinensis was 61.29%,and the infection rate of Theileria luwenshuni was 20.16%.The results in this study showed that the infection rate of Piroplasma in yak was high in Ganzi prefecture,and was different among regions,the predominant species were Theileria sinensis and Theileria luwenshuni,contributing to the prevention and control of piroplasmosis in yak in Ganzi prefecture. |
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| Keywords: | Piroplasma infection condition yak |
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