| 人工合成小麦SHW-L1高硒含量KASP分子标记开发及其应用 |
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| 引用本文： | 魏广辉,李执,陈强,李阳,陈诗豪,裴英,周勇,程梦萍,唐豪,王际睿,魏育明,刘登才,陈黎,郑有良,蒲至恩.人工合成小麦SHW-L1高硒含量KASP分子标记开发及其应用[J].中国农业科学,2020,53(20):4103-4112. |
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| 作者姓名： | 魏广辉 李执 陈强 李阳 陈诗豪 裴英 周勇 程梦萍 唐豪 王际睿 魏育明 刘登才 陈黎 郑有良 蒲至恩 |
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| 作者单位： | 1西南作物基因资源发掘与利用国家重点实验室,成都6111302四川农业大学农学院,成都6111303四川农业大学小麦研究所,成都6111304宜宾市产品质量监督检验所,四川宜宾644000 |
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| 基金项目： | 国家重点研发计划(2017YFD0100900);四川省教育厅重点项目(17ZA0315) |
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| 摘 要： | 【目的】硒作为人体必不可少的元素之一,在疾病预防与抗氧化方面具有重要作用。小麦作为主要作物之一,是人类获取植物性硒源的主要来源,因此,提高小麦籽粒硒含量是人体补充硒的一条高效、低廉、简单易行的途径。拟通过对已定位到的SNP位点开发KASP标记用于开展高硒小麦选育。【方法】利用已经完成的660K小麦SNP标记的人工合成小麦SHW-L1重组自交系（recombinant inbred lines,RILs）群体,将前期定位的籽粒硒含量相关的QTL区间进一步缩小,开发稳定基因内的SNP位点为KASP标记。【结果】成功开发出2个KASP标记AX-1和AX-2,标记能够在群体中进行基因型分型和鉴定材料籽粒硒含量的相对高低。2个标记都可将供试材料按照基因型分为2类,即高硒和低硒材料,符合1:1的等位基因分离比例。但按照表现型来划分,标记对低硒材料的筛选率（大于90%）要远高于高硒材料（小于30%）。具有高硒含量的表现型材料中,有81%的材料具有高硒的基因型,表明了标记的可靠性。同时,在以人工合成小麦SHW-L1为亲本的其他衍生后代株系中也能用该KASP标记进行基因型分型,分型结果与表型结果一致,说明了标记的有效性,也表明可通过开发到的KASP标记来提升选择效率。【结论】AX-1和AX-2可作为一个实用的分子标记用于SHW-L1为亲本的高硒小麦品种选育和种质资源创制。
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| 关 键 词： | 人工合成小麦 硒含量 KASP标记 |
| 收稿时间： | 2019-12-02 |
Development and Utilization of KASP Marker for Se Concentration in Synthetic Wheat SHW-L1 |
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| WEI GuangHui,LI Zhi,CHEN Qiang,LI Yang,CHEN ShiHao,PEI Ying,ZHOU Yong,CHENG MengPing,TANG Hao,WANG JiRui,WEI YuMing,LIU DengCai,CHEN Li,ZHENG YouLiang,PU ZhiEn.Development and Utilization of KASP Marker for Se Concentration in Synthetic Wheat SHW-L1[J].Scientia Agricultura Sinica,2020,53(20):4103-4112. |
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| Authors: | WEI GuangHui LI Zhi CHEN Qiang LI Yang CHEN ShiHao PEI Ying ZHOU Yong CHENG MengPing TANG Hao WANG JiRui WEI YuMing LIU DengCai CHEN Li ZHENG YouLiang PU ZhiEn |
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| Institution: | 1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 6111302College of Agronomy, Sichuan Agricultural University, Chengdu 6111303Triticeae Research Institute, Sichuan Agricultural University, Chengdu 6111304Yibin Product Quality Supervision and Inspection Institute, Yibin 644000, Sichuan |
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| Abstract: | 【Objective】As one of the essential elements of the human body, selenium plays an important role in disease prevention and antioxidants. Wheat, as one of the main crops, is the main source for humans to obtain plant selenium sources, so increasing the selenium content of wheat grains is an efficient, inexpensive and easy way to supplement selenium. This study intends to develop KASP markers for the identified SNP loci for the breeding of high-selenium wheat.【Method】Using the completed 660K wheat SNP markers of synthetic wheat SHW-L1 recombinant inbred lines population ( recombinant inbred lines, RILs), scanning the RILs population, further narrowing the QTL interval related to the selenium content of the previously located grains, and developing the SNP loci in the stable gene as KASP markers.【Result】Two successfully developed KASP markers AX-1 and AX-2, markers can be used for genotyping and identification of selenium content in the grain of the material. Both markers can be used to classify test materials according to genotype into two categories, namely high-selenium and low-selenium materials, meets the 1:1 allele separation ratio. However, according to the phenotype, the screening rate (greater than 90%) for low-selenium materials is much higher than that of high-selenium materials (less than 30%), and it should be clear that 81% of the phenotype materials with high selenium content have high selenium genotypes, indicating the reliability of the label. Therefore, the KASP markers developed can be used to improve selection efficiency. At the same time, the KASP marker can also be used for genotyping in other derived offspring lines with artificial wheat SHW-L1 as the parent, and the typing results are consistent with the phenotypic results, indicating the validity of the marker.【Conclusion】AX-1 and AX-2 can be used as a practical molecular marker for breeding and germplasm resource creation of high selenium wheat varieties with SHW-L1 as the parent. |
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| Keywords: | synthetic wheat selenium content KASP |
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