| H_2O_2对葡萄离体叶片白藜芦醇的诱导作用 |
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| 引用本文： | 黄方爱,张波,杨晓燕,颜欢,陈韩英,郑秋生.H_2O_2对葡萄离体叶片白藜芦醇的诱导作用[J].新疆农业科学,2012(10):1799-1804. |
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| 作者姓名： | 黄方爱 张波 杨晓燕 颜欢 陈韩英 郑秋生 |
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| 作者单位： | 石河子大学药学院;省部共建新疆特种资源植物药重点实验室 |
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| 基金项目： | 国家自然科学基金项目(30860032;31160058);兵团博士资金项目(2011BB018) |
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| 摘 要： | 【目的】葡萄叶片白藜芦醇含量较虎杖根含量低,难以作为药用提取用途,研究采用诱导剂升高葡萄离体叶片中白藜芦醇的含量。【方法】实验采用H_2O_2对离体红地球葡萄叶片进行诱导,采用薄层及高效液相色谱法分析白藜芦醇的含量变化,并使用RT-PCR方法分析芪合酶基因(STS)的表达量变化。【结果】H_2O_2对离体叶片芪类物质具有显著的诱导作用,诱导白藜芦醇的积累呈现显著的量时依赖性;5%H_2O_2处理48 h后叶片白藜芦醇含量可提高15倍以上,鲜重达到263μg/g。【结论】H_2O_2可以作为葡萄离体叶片白藜芦醇的有效诱导剂。
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| 关 键 词： | 白藜芦醇 过氧化氢 葡萄叶片 茋合酶 |
The Induction of Hydrogen Peroxide on Resveratrol in Detached Grape Leaves |
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| Institution: | HUANG Fang - ai1,ZHANG Bo1,YANG Xiao - yan1,YAN Huan1,CHEN Han - ying1,ZHENG Qiu - sheng2 (1.College of Pharmacy,Shihezi University,Shihezi Xinjiang 832002,China;2.Key Laboratory of Xinjiang Endemic Phytomedicine Resources,Ministry of Education,College of Pharmacy,Shihezi University, Shihezi Xinjiang 832002,China) |
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| Abstract: | Objective]It is hard to extract resveratrol from grape leaves in a medicinal usage because resveratrol in normal grape leaves is lower than in Polygonum cuspidatum root.So present investigation aims to induce resveratrol accumulations in the detached grape leaves.Method]In the present study,the detached grape leaves(Vitis vinifera L.cv.Red Globe) were treated with H_2O_2 solution to investigate the changes of resveratrol content by TLC and HPLC methods.The mRNA expression level of stilbene synthase(STS) was also checked in the treatments.Result]The results showed that stilbenes accumulated significantly while the resveratrol were induced in both dose and time dependent manners in H_2O_2 - treated leaves.Further,the maximum content of resveratrol reached 263μg/g in treated leaves by 5%H_2O_2 after 48 h,which was 15 - fold higher than the untreated.Conclusion]Therefore,H_2O_2 is confirmed as an effective resveratrol inducer to facilitate the use of the detached leaves. |
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| Keywords: | resveratrol hydrogen peroxide grape leaf stilbene synthase |
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