| 不同玉米自交系幼胚愈伤组织诱导和分化的研究 |
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| 引用本文： | 林霞,王志安,陈玲,李新鹏,吴家和,夏春兰,胡保民,陈全家,王沛政.不同玉米自交系幼胚愈伤组织诱导和分化的研究[J].新疆农业科学,2012,49(9):1587-1593. |
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| 作者姓名： | 林霞 王志安 陈玲 李新鹏 吴家和 夏春兰 胡保民 陈全家 王沛政 |
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| 作者单位： | 新疆农业大学农学院,乌鲁木齐,830052;新疆康地种业科技股份有限公司,乌鲁木齐,830011;中国科学院微生物研究所,北京,100101 |
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| 基金项目： | 中国科学院科技支新项目(Y02B011HH1) |
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| 摘 要： | 目的]通过对玉米幼胚培养,建立一套稳定性好、再生率高的组织培养体系.方法]以4个玉米自交系幼胚为外植体,研究不同基因型、幼胚大小、2,4-D浓度、AgN03浓度对愈伤组织诱导的影响,进一步研究6 - BA和蔗糖浓度对愈伤组织分化及IBA和NAA对再生苗的生根影响.结果]2,4-D浓度为2.0 ～3.0mg/L,幼胚长度为1.0～2.0 mm时,对胚性愈伤组织诱导有良好效果；在诱导期间采用隔代法添加10 mg/LAgNO3能提高胚性愈伤组织的诱导率；当6- BA为0.5 mg/L、蔗糖浓度为50 g/L时,愈伤组织分化率最高且有利于小植株的形成；IBA浓度为0.6 mg/L对植株生根较有利.结论]对4个玉米自交系幼胚培养研究筛选出适宜玉米幼胚培养的最佳诱导、分化及生根培养基.
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| 关 键 词： | 玉米 幼胚 愈伤组织 植株再生 |
| 收稿时间： | 2012-09-25 |
Study on Callus Induction and Differentiation of Immature Embryos from the Various Maize Inbred Lines |
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| LIN Xia,WANG Zhi-an,CHEN ling,LI Xin-peng,WU Jia-he,XIA Chun-lan,HU Bao-min,CHEN Quan-jia,WANG Pei-zheng.Study on Callus Induction and Differentiation of Immature Embryos from the Various Maize Inbred Lines[J].Xinjiang Agricultural Sciences,2012,49(9):1587-1593. |
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| Authors: | LIN Xia WANG Zhi-an CHEN ling LI Xin-peng WU Jia-he XIA Chun-lan HU Bao-min CHEN Quan-jia WANG Pei-zheng |
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| Institution: | LIN Xia1,WANG Zhi - an2,CHEN ling2,LI Xin - peng2,WU Jia - he3,XIA Chun - lan2, HU Bao - min2,CHEN Quan - jia1,WANG Pei - zheng2 (1.College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;2.Xinjiang Kangdi Seed Science & Technology Co.Ltd.,Urumqi 830011,China;3.Microbiology Institute of Chinese Academy of Sciences,Beijing 100101,China) |
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| Abstract: | Objective]The purpose of this research was to develop a stable protocol of high frequent plantlet regeneration via culturing of immature embryos in maize.Method]The four inbred lines of maize were collected as experiment materials.The affection of different of genotypes,immature embryo size,2,4 - D concentration and AgNO_3 concentration on callus induction were analyzed.Further affection of different concentration of 6 - BA and sucrose on callus differentiation and the various combinations of IBA and NAA on regeneration of plantlets were studied as well.Result]The length of 1.0 -2.0 mm immature embryo had better effect on callus induction and differentiation than other sizes.The induction rate of embryogenic callus can be improved by adding 10mg / L AgNO_3 on an interval generation during induced period.When the concentration of 6 - BA was 0.5 mg/L and sucrose was 50 g/L,the rate of callus differentiation was the highest and the plantlets were easy to form.The concentration 0.6 mg / L of IBA were more favorable for plant root formation.Conclusion]Through studies of immature embryo of the 4 inbred lines of maize culture,the optimal culture of immature embryo,differentiation and rooting were available in maize. |
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| Keywords: | maize immature embryo callus plant regeneration |
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