| 茄类内生菌的分离及拮抗细菌的筛选 |
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| 引用本文： | 刘杰凤,韩寒冰,张进凤,罗添喜,詹加钦.茄类内生菌的分离及拮抗细菌的筛选[J].农业科学与技术,2009,10(1):35-38. |
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| 作者姓名： | 刘杰凤 韩寒冰 张进凤 罗添喜 詹加钦 |
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| 作者单位： | 茂名学院化学与生命科学学院,广东茂名,525000
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| 摘 要： | 1材料与方法
1．1植株及病原菌 健康的番茄、辣椒及茄子植株，采自茂名市郊区菜园；茄科雷尔氏菌（Ralstonia solanacearum）由茂名学院生物技术与食品工程实验中心分离得到。
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| 关 键 词： | 拮抗细菌 分离 番茄 内生菌 筛选 实验中心 食品工程 生物技术 |
Isolation of Endophyte and Screening for Antagonistic Bacteria in Solanaceae |
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| LIU Jie-feng,HAN Han-bing,ZHANG Jin-feng,LUO Tian-xi,ZHAN Jia-qin.Isolation of Endophyte and Screening for Antagonistic Bacteria in Solanaceae[J].Agricultural Science ＆ Technology,2009,10(1):35-38. |
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| Authors: | LIU Jie-feng HAN Han-bing ZHANG Jin-feng LUO Tian-xi ZHAN Jia-qin |
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| Institution: | ( College of Chemistry and Life Science, Maoming University, Maoming 525000) |
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| Abstract: | Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. Method] According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified endophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae. |
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| Keywords: | Endophyte Isolation Screening Antagonism |
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