| 应用RT-PCR方法检测水稻植株和介体昆虫体内的水稻齿叶矮缩病毒 |
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| 引用本文： | 雷娟利,吕永平,金登迪,陈声祥,陈剑平.应用RT-PCR方法检测水稻植株和介体昆虫体内的水稻齿叶矮缩病毒[J].植物病理学报,2001,31(4):306-309. |
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| 作者姓名： | 雷娟利 吕永平 金登迪 陈声祥 陈剑平 |
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| 作者单位： | 浙江省农业科学院病毒室, 杭州 310021 |
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| 基金项目： | 美国洛氏基金(RF98001#68);浙江省青年科技人才专项基金(RC9604);浙江省重点实验室专项(01107557) |
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| 摘 要： | 将生物学接种和RT-PCR检测水稻植株体内的水稻齿叶矮缩病毒的方法进行了比较,发现结果基本趋于一致,但总体上RT-PCR检测阳性率稍高于生物学接种的发病率。将生物学接种检测褐飞虱介体内水稻齿叶矮缩病毒方法与RT-PCR检测方法进行比较分析,发现RT-PCR方法可以检测单头褐飞虱体内的水稻齿叶矮缩病毒,具有很高的准确性和灵敏度。
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| 关 键 词： | 水稻齿叶矮缩病毒 R T-P CR检测 生物学接种 症状 |
| 文章编号： | 0412-0914(2001)04-0306-04 |
| 修稿时间： | 2000年4月20日 |
RT-PCR DETECTON OF Rice ragged stunt virus IN RICE PLANT AND INSECT VECTOR |
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| LEI Juan li,LU Yong ping,JIN Deng di,CHEN Sheng xiang,CHEN Jian ping.RT-PCR DETECTON OF Rice ragged stunt virus IN RICE PLANT AND INSECT VECTOR[J].Acta Phytopathologica Sinica,2001,31(4):306-309. |
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| Authors: | LEI Juan li LU Yong ping JIN Deng di CHEN Sheng xiang CHEN Jian ping |
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| Institution: | Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | RT PCR was compared with biological method in detecting Rice ragged stunt virus (RRSV) in rice plants. Results indicated that RT PCR method gave corresponding result to that of biological inoculation, but in general, RT PCR method was more sensitive. Compared with detection of the RRSV in insect vector by virus transmission, RT PCR was also more accurate and the RRSV could be detected even in a single vector. |
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| Keywords: | Rice ragged stunt virus RT PCR detection biological inoculation symptom |
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