Study of equid herpesviruses 2 and 5 in Iceland with a type-specific polymerase chain reaction |
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| Institution: | 1. Clinic of Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Germany;2. Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany;3. Department of Pediatric Pulmonology and Neonatology, Hannover Medical School, Hannover, Germany;4. Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany;1. Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy;2. Multiple Sclerosis Center, Hospital ‘S. Antonio Abate’, Gallarate, VA, Italy;3. Neurological Department, Valduce Hospital, Como, Italy;4. Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy;1. Section of Gynecologic Oncology, Department of Surgical Oncology, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA;2. Division of Gynecologic Oncology, Hospital of the University of Pennsylvania, 3rd Floor, PCAM, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA;3. Department of Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, 501 Blockley Hall, 423 Guardian Drive, Philadelphia, PA 19104, USA;4. Women''s Cancer Research Institute at the Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA;5. Division of Gynecologic Oncology, University of New Mexico Cancer Center, 1201 Camino de Salud NE, 1 University of New Mexico, Albuquerque, NM 87106, USA |
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| Abstract: | The horse population in Iceland is a special breed, isolated from other horses for at least 1000 years. This provides an exceptional opportunity to investigate old and new pathogens in an inbred herd with few infectious diseases. We have developed a high sensitivity semi-nested PCR to study equid gammaherpesviruses 2 and 5 (EHV-2 and 5) in Iceland. The first PCR is group specific, the second type-specific, targeting a 113 bp sequence in the glyB gene. DNA isolated from white blood cells and 18 different organs was tested for the presence of EHV-2 and 5. This was done in adult horses and foals, healthy and with various enteric infections. Both virus types were easily detected in all types of organs tested or EHV-2 in 79% cases and EHV-5 in 63%. In DNA from PBMC or buffy-coat EHV-2 was found in 20% cases and EHV-5 in 10%, all except one positive were foals. Co-culture of PBMC on fetal horse kidney cells was efficient for detecting EHV-2 but not for EHV-5. We verify here for the first time infections with EHV-2 and 5 in horses in Iceland and show that both viruses are common. |
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