| 烟草青枯病菌分离株RSXJ-1的培养性状及其生物型鉴定 |
| |
| 引用本文： | 崔朝宇,张超群,周泽科,蒋军喜.烟草青枯病菌分离株RSXJ-1的培养性状及其生物型鉴定[J].江西植保,2012(2):153-156. |
| |
| 作者姓名： | 崔朝宇 张超群 周泽科 蒋军喜 |
| |
| 作者单位： | 1. 江西农业大学 农学院,江西 南昌 330045
2. 江西烟叶科学研究所,江西 南昌 330025 |
| |
| 基金项目： | 江西省烟草专卖局(公司)资助项目(201001021) |
| |
| 摘 要： | 从江西省峡江县烟区采集呈典型症状的青枯病烟株,采用平板梯度稀释分离法分离获得一株病原菌分离株,命名为RSXJ-1。在完成致病性测定的基础上,通过分子生物学方法将该菌株鉴定为茄科劳尔氏菌(Ralstonia solanacearum)。本文研究了该菌株在不同培养基上的培养性状,结果表明该菌株的培养性状与R.solanacearum的培养性状相一致;对该菌株进一步进行生物型研究,结果显示该菌株能利用乳糖、麦芽糖、纤维二糖、甘露醇和山梨醇氧化产酸并能还原硝酸盐;而不能利用甜醇氧化产酸,据此将该菌株鉴定为生物型Ⅲ-1。
|
| 关 键 词： | 烟草青枯病菌 培养性状 生物型 |
Cultural Characteristics and Biotype Identification of Isolate RSXJ-1 of Ralstonia solanacearum |
| |
| CUI Chao-yu,ZHANG Chao-qun,ZHOU Ze-ke,JIANG Jun-xi.Cultural Characteristics and Biotype Identification of Isolate RSXJ-1 of Ralstonia solanacearum[J].Jiangxi Plant Protection,2012(2):153-156. |
| |
| Authors: | CUI Chao-yu ZHANG Chao-qun ZHOU Ze-ke JIANG Jun-xi |
| |
| Institution: | 1(1.College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China;2.Jiangxi Tobacco Science Research Institute,Nanchang 330025,China) |
| |
| Abstract: | A tobacco plant with typical symptoms of bacterial wilt was collected from Xiajiang county of Jiangxi province.A bacterial isolate was obtained from the plant using gradient dilution isolation method,ant it was named RSXJ-1.With completion of its pathogenicity test,the isolate was identified molecularly as Ralstonia solanacearum.This paper investigated the cultural characteristics of the bacterial isolate on different media.The results showed that cultural characteristics of the bacterial isolate were consistent with the cultural characteristics of R.solanacearum.Experiment of biotype identification of the isolate was also conducted.The results showed that the isolate had the ability of both oxdizing lactose,maltose,cellobiose,mannitol,and sorbitol to produce acid and reducing nitrate.However,it had no ability of oxdizing dulcitol to produce acid.According to the results,the isolate was identified as biotype Ⅲ-1 of R.solanacearum. |
| |
| Keywords: | Ralstonia solanacearum cultural characteristics biotype |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
