| 青钱柳叶总黄酮大孔树脂纯化工艺 |
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| 引用本文： | 吴彩娥,方升佐,冯宗帅,杨万霞,李婷婷,贾韶千.青钱柳叶总黄酮大孔树脂纯化工艺[J].农业机械学报,2009,40(6). |
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| 作者姓名： | 吴彩娥 方升佐 冯宗帅 杨万霞 李婷婷 贾韶千 |
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| 作者单位： | 南京林业大学森林资源与环境学院,南京,210037 |
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| 摘 要： | 为提高青钱柳叶总黄酮的纯度,筛选适宜青钱柳叶总黄酮纯化的大孔树脂,确定青钱柳叶总黄酮纯化工艺参数,选用大孔树脂材料,采用静态与动态吸附-解吸方法对青钱柳叶总黄酮进行纯化,以吸附量与洗脱率为考察指标对树脂进行选择与工艺研究,并利用紫外可见分光光度计测量青钱柳叶总黄酮的含量.结果表明:AB-8型大孔树脂纯化效果最好,其最佳工艺为进样速度2 BV/h、进样质量浓度1.0 mg/mL,洗脱剂最适宜体积分数与洗脱速度分别为70%和2 BV/h.表明AB-8型大孔树脂适合青钱柳叶总黄酮的纯化.
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| 关 键 词： | 总黄酮 青钱柳叶 大孔树脂 纯化 |
Purification Process of Total Flavonoids in Cyclocarya paliurus Leaves with Macroporous Resins |
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| Wu Caie,Fang Shengzuo,Feng Zongshuai,Yang Wanxia,Li Tingting,Jia Shaoqian.Purification Process of Total Flavonoids in Cyclocarya paliurus Leaves with Macroporous Resins[J].Transactions of the Chinese Society of Agricultural Machinery,2009,40(6). |
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| Authors: | Wu Caie Fang Shengzuo Feng Zongshuai Yang Wanxia Li Tingting Jia Shaoqian |
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| Institution: | College of Forest Resources and Environment;Nanjing Forestry University;Nanjing 210037;China |
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| Abstract: | With the aim to improve the purity of total flavonoids of Cyclocarya paliurus leaves,the suitable resin for separating and purifying total flavonoids of Cyclocarya paliurus leaves and the optimum parameters of purification were studied.The optimal techniques of separation and purification of flavonoid were obtained by static adsorption and elution tests,and the separation techniques were evaluated by measuring the concentration of total flavonoids of Cyclocarya paliurus leaves with ultraviolet spectrophotom... |
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| Keywords: | Total flavonoids Cyclocarya paliurus leaves Macroporous resin Purification |
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