| 铜、锰诱导吉富罗非鱼血细胞凋亡及铁、锌的干预作用 |
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| 引用本文： | 白丽蓉,赵志英.铜、锰诱导吉富罗非鱼血细胞凋亡及铁、锌的干预作用[J].中国农学通报,2015,31(35):58-65. |
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| 作者姓名： | 白丽蓉 赵志英 |
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| 作者单位： | 海南省海洋与渔业科学院,海南省海洋与渔业科学院 |
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| 基金项目： | 海南省科学事业费项目“海水养殖对海南沿岸生态环境影响及控制措施研究”(11-201410-0009)。 |
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| 摘 要： | 为了研究重金属铜与锰对吉富罗非鱼血细胞凋亡的影响以及铁、锌的拮抗作用，采用原子吸收分光光度法检测血液及饲料中重金属的含量，采用流式细胞术检测实验鱼血细胞凋亡情况。360 尾吉富罗非鱼幼鱼随机分为12 组，每组30 尾，分别以硫酸铜(0、2000 mg/kg)、硫酸锰(0、120 mg/kg)为攻毒组重金属源，以硫酸锌(20、320 mg/kg)、硫酸铁(150、350 mg/kg)为拮抗组金属源，通过饲料投喂的方式进行血细胞凋亡的研究，养殖周期为20 周。细胞凋亡分析结果表明，染铜组、染锰组实验鱼血细胞凋亡率显著高于对照组；补充铁和锌后，随着饲料添加铁、锌水平的增加，实验鱼血细胞凋亡率明显下降，但铁、锌干预组血细胞凋亡率显著高于对照组。实验表明，过量铜、锰可诱发实验罗非鱼血细胞凋亡；较高水平的铁、锌对铜、锰的毒性作用具有拮抗作用。
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| 关 键 词： | 土壤水分 土壤水分 热惯量法 能量指数法 植被供水指数法 |
| 收稿时间： | 2015/5/11 0:00:00 |
| 修稿时间： | 2015/11/17 0:00:00 |
Effects of Copper and Manganese on Hemocyte Apoptosis and the Antagonism of Iron and Zinc in Oreochromis nilotcus |
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| Abstract: | The objective of this study was to investigate the influence of copper and manganese on apoptosis in blood cells of Oreochromis nilotcus and the antagonistic effect of iron and zinc. Atomic absorption spectrophotometry was used to analyze heavy mental content in fish blood and food, and flow cytometry was applied to analyze fish blood cell apoptosis. Juvenile Tilapia were randomly divided into 12 groups with 30 fish in each group. The challenge groups were fed with constant level of copper sulfate (0, 2000 mg/kg) and manganese sulfate (0, 120 mg/kg) diet, and the antagonized groups were fed with zinc sulfate (20, 320 mg/kg) and iron sulfate (150, 350 mg/kg). Twenty weeks later, the number of apoptotic cells in the blood obviously increased in copper group and manganese group. After adding iron and zinc, the number of apoptotic cells was lower than that of the copper group and manganese group. The numbers of apoptotic cells in the iron and zinc interventional groups were significantly higher than that in the control group. Copper and manganese can cause apoptosis in blood cells. However, those effects can be antagonized by iron and zinc. |
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| Keywords: | Oreochromis nilotcus Apoptosis Copper Manganese Iron Zinc |
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