| 盐度梯度介导mTOR信号通路对尼罗罗非鱼生长的影响 |
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| 引用本文： | 王飞,庄青青,梁从飞,颜标,赵金良.盐度梯度介导mTOR信号通路对尼罗罗非鱼生长的影响[J].上海海洋大学学报,2014,23(6):820-825. |
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| 作者姓名： | 王飞 庄青青 梁从飞 颜标 赵金良 |
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| 作者单位： | 农业部淡水水产种质资源重点实验室,农业部淡水水产种质资源重点实验室,农业部淡水水产种质资源重点实验室,农业部淡水水产种质资源重点实验室,农业部淡水水产种质资源重点实验室 |
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| 基金项目： | 现代农业产业技术体系专项资金（CARS-49-4B） |
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| 摘 要： | mTOR信号通路是动物生长发育的重要调控通路之一。以尼罗罗非鱼为实验材料,研究了不同盐度梯度对尼罗罗非鱼生长的影响,以及mTOR通路下游调节因子p70s6k和4ebp1的mRNA与蛋白表达水平变化。30 d养殖结果显示,盐度增加对尼罗罗非鱼生长具有明显抑制作用,0、15、20、25盐度下,尼罗罗非鱼日均增重率分别为(0.48±0.13)g/d、(0.31±0.09)g/d、(0.14±0.08)g/d、(0.09±0.03)g/d。荧光定量PCR显示,p70s6k mRNA相对表达量随着盐度梯度升高而下降,0盐度组中表达量最高,显著高于其他盐度组(P0.05),25盐度组最低;4ebp1 mRNA相对表达量随着盐度梯度升高而升高,0盐度组中表达量最低,25盐度组最高,0盐度组与25盐度组间差异显著(P0.05)。Western blot结果显示,0盐度组中p70s6k蛋白含量显著大于其他高盐度组,而25盐度组中4ebp1蛋白含量显著大于其余低盐度组(P0.05)。结果表明,盐度可能通过介导mT OR信号通路参与尼罗罗非鱼的生长调节。
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| 关 键 词： | 尼罗罗非鱼 盐度 生长 p70s6k 4ebp1 |
| 收稿时间： | 2014/3/12 0:00:00 |
| 修稿时间： | 2014/5/19 0:00:00 |
Effects of salinity on growth of Oreochromis niloticus through mTOR signal pathway |
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| WANG Fei,ZHUANG Qing-qing,LIANG Cong-fei,YAN Biao and ZHAO Jin-liang.Effects of salinity on growth of Oreochromis niloticus through mTOR signal pathway[J].Journal of Shanghai Ocean University,2014,23(6):820-825. |
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| Authors: | WANG Fei ZHUANG Qing-qing LIANG Cong-fei YAN Biao and ZHAO Jin-liang |
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| Institution: | Laboratory of Freshwater Fisheries Germplasm Resources,Ministry of Agriculture,Shanghai Ocean University,Laboratory of Freshwater Fisheries Germplasm Resources,Ministry of Agriculture,Shanghai Ocean University,Laboratory of Freshwater Fisheries Germplasm Resources,Ministry of Agriculture,Shanghai Ocean University,Laboratory of Freshwater Fisheries Germplasm Resources,Ministry of Agriculture,Shanghai Ocean University,Laboratory of Freshwater Fisheries Germplasm Resources,Ministry of Agriculture,Shanghai Ocean University |
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| Abstract: | mTOR signal pathway plays a vital role in the regulation animal growth and development. In this study, growth rate of Oreochromis niloticus under different salinities were compared in 30 days experiment, meantime, expression change of downstream pathway regulation factors p70s6k and 4ebp1 were evaluated at both mRNA and protein level. The results showed that the final body weight decreased with the salinity increasing, average growth weight at 0g/l, 15g/l, 20g/l, 25g/l salinities was 0.48±0.13g/d, 0.31±0.09g/d, 0.14±0.08g/d, 0.09±0.03g/d, respectively. Salinity inhibited the growth of Nile tilapia. p70s6k mRNA expression level decreased with salinity increasing, the highest was detected in 0g/l salinity, while the lowest was in 25g/l salinity, which was significant lower than in 0g/l, 15g/l and 20g/l salinities. 4ebp1 mRNA expression at 25g/l salinity was significantly higher than 0g/l, 15g/l, 20g/l, it increased with salinity levels. Western blot results showed the protein level of p70s6k at 0g/l salinity was significantly higher than other higher salinities, while 4ebp1 at 25g/l salinity was significantly higher than other lower salinities. Our result suggested salinity may affect growth of O. niloticus though mTOR signaling pathway. |
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| Keywords: | Oreochromis niloticus Salinity Growth P70s6k 4ebp1 |
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