| 猪源大肠杆菌O157:H7广西分离株的鉴定 |
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| 引用本文： | 禤雄标,陈泽祥,谢永平,胡帅,许力干,杨威.猪源大肠杆菌O157:H7广西分离株的鉴定[J].中国动物检疫,2010,27(3):52-53. |
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| 作者姓名： | 禤雄标 陈泽祥 谢永平 胡帅 许力干 杨威 |
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| 作者单位： | 广西壮族自治区兽医研究所,广西南宁,530001 |
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| 基金项目： | 广西区科技厅攻关项目 |
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| 摘 要： | 为了解大肠杆菌O157:H7是否在猪场存在,采集有拉稀症状猪的粪便,接种到新生霉素mEC增菌肉汤,增菌培养后转接到O157:H7显色培养基上,对可疑菌株用因子血清和PCR方法做进一步鉴定,实验结果证实得到5株大肠杆菌为O157:H7血清型。动物试验发现,5个分离菌株对小白鼠的致死率为33.3%—100%之间。调查结果证实广西猪群中存在强致病性的O157:H7血清型大肠杆菌。
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| 关 键 词： | 大肠杆菌O157:H7 分离鉴定 PCR方法 毒力实验 |
Identification of pigs Escherichia Coli O157 isolates obtained in Guangxi province of china |
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| Xuan Xiongbiao,Chen Zexiang,Xie Yongping,Hu Shuai,Xu Ligan,Yang Wei.Identification of pigs Escherichia Coli O157 isolates obtained in Guangxi province of china[J].China Journal Of Animal Quarantine,2010,27(3):52-53. |
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| Authors: | Xuan Xiongbiao Chen Zexiang Xie Yongping Hu Shuai Xu Ligan Yang Wei |
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| Institution: | (Guangxi Veterinary Research Institute,Nanning 530001,China) |
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| Abstract: | In order to investigate whether or not Escherichia Coli O157 infection in domestic pigs in Guangxi farms,Samples of fresh faeces or faecal swab were collected and cultured by enriching in novobiocin mEC broth ,and transferred to O157 chromogenic medium,those suspicious strains were further identified with specificity antiserum and PCR.finally,The result confirmed five different strains were Escherichia Coli O157:H7 ,Animal study showed that these strains’s death rate for mouse between 33.3% and 100%,the investigation indicted that strong pathogenicity Escherichia Coli O157:H7 strain existed in domestic pigs in Guangxi farms. |
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| Keywords: | EC O157:H7 isolation and identification PCR toxic experiment |
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