| 苏木水提物抗蛇毒磷脂酶A2活性研究 |
| |
| 作者姓名： | 乐贞 陈旭明 姚水洪 冯敬骞 朱晓萍 陈敏 |
| |
| 作者单位： | 衢州职业技术学院医学院 |
| |
| 摘 要： | 中药苏木(Sappan Lignum)来源于植物苏木(Caesalpinia sappan L.)的干燥心材，具有止血
抗炎等功效。采用琼脂糖平板法研究不同浓度的苏木水提物（0.03g/ml，0.06g/ml，0.09g/ml，
0.12g/ml，0.15g/ml）对蛇毒sPLA2水解活性的抑制作用，测量透明圈和孔的直径，根据酶活标准曲
线计算其抑制率。结果表明0.15g/ml 苏木水提物对五步蛇毒sPLA2水解活性有抑制作用。
|
| 关 键 词： | 苏木 五步蛇毒 抗蛇毒活性 |
Study on the water extract of Sappa Lignum against the activity of
phospholipase A2 from Snake Venom |
| |
| Authors: | Le Zhen Chen Xuming Yao Shuihong Feng Jingqian Zhu Xiaoping Chen Min |
| |
| Institution: | QuZhou College of Technology ZheJiang |
| |
| Abstract: | The traditional Chinese medicine Sappan Lignum is derived from the dry heartwood of the
plant Caesalpinia sappan L. It has the effect of hemostasis and anti-inflammation. Agarose plate meth?
od was used to study the inhibitory effect of the water extract of Sappa Lignum (0.03g / ml, 0.06g / ml,
0.09 g / ml, 0.12g / ml, 0.15g / ml) on the hydrolysis activity of snake venom sPLA2. The diameter of
transparent circle and pore were measured and the inhibition rate was calculated according to the stan?
dard curve of enzyme activity. The results show that the water extract of 0.15g/ml has the inhibitory ac?
tivity against the hydrolysis activity of sPLA2 from the venom of Agkistrodon acutus. |
| |
| Keywords: | sappan lignum agkistrodon acutus venom antivenom activity |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
