| 大豆花药愈伤组织的分化及其内源激素分析 |
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| 引用本文： | 叶兴国,王连铮.大豆花药愈伤组织的分化及其内源激素分析[J].作物学报,1997,23(5):555-561. |
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| 作者姓名： | 叶兴国 王连铮 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所，北京，100081 |
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| 摘 要： | 选用３１个栽培大豆基因型进行花药培养。愈伤组织诱导率２．２％￣３６．６％，８个基因型的出愈率在２５％以上，６个基因型产生了芽或胚状体，只有丰收黄、鲁豆１０呈二个基因型既产生了芽，又产生了胚状体，具有相对高的培养力。３年内共产生了１４个再生芽、９个胚状体、６个芽状物和一个根、芽齐全的小再生植株。虽然获得花粉植株属于１５年来的第一例，但愈伤组织分化率仍然很低，这与愈伤组织的状态和质量较差有很大关系。愈
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| 关 键 词： | 大豆 花药培养 愈伤组织分化 胚状体 内源激素 |
| 收稿时间： | 1996-04-19 |
| 修稿时间： | 1996-12-13 |
Differentiation of Callus and Analysis of Endogenous Hormone in Anther Culture of Soybean(Glycine max) |
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| Ye Xingguo,Wang Lianzheng.Differentiation of Callus and Analysis of Endogenous Hormone in Anther Culture of Soybean(Glycine max)[J].Acta Agronomica Sinica,1997,23(5):555-561. |
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| Authors: | Ye Xingguo Wang Lianzheng |
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| Institution: | Institute of Crop Breeding and Cultivation, CAAS, Beijing,100081 |
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| Abstract: | Soybean (Clycine max)is a recalcitrant crop for in vitro regeneration and the nature makes the progress in haploid plant induction sluggish. Thrity-one soybean genotypes were used in this study of anther culture and screened for high inducing ability. Relation between callus regeneration and endogenous hormone was analysed. Calli were induced from the anthers with a range of 2. 2 to 36. 6 percent. Eight genotypes were screened for their high percentage of callus formation, over 25 percent. Shoots or embryoids regenerated from the calli of six genotypes, among which the calli derived from Fengshouhuang and Ludou 10 differentiated not only shoots, but also embryoids, with relatively sensitive anther culture response. Fourteen shoots, nine embryoids, and six bud-like structures were obtained in three years from six genotypes. For the first time, a plantlet with roots was achieved in the world within the later fifteen years. In spite of the above, the callus regeneration still was very difficult and no reproducible, which is probably related to the low quality of the callus.No ABA and ZT that were essential to differentiation of callus were tested in the calli or shoots, embryoids, which may be the main reason of difficult differentiation of the callus or easy withering of the shoot. After adding ABA in differentiation medium, no ABA still was examined in the calli. So, the leveling and harmonizing of endogeneous hormone should be paid attention to from the begining of callus induction for improving the callus quality. |
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| Keywords: | Glycine max Anther culture Callus differentiation Embryoid Endogenous hormone |
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