| 暗纹东方中BAFF基因相关序列的性质探讨 |
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| 引用本文： | 魏翠,余多慰.暗纹东方中BAFF基因相关序列的性质探讨[J].安徽农业科学,2008,36(10). |
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| 作者姓名： | 魏翠 余多慰 |
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| 作者单位： | 南京师范大学生命科学学院,江苏南京,210046 |
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| 基金项目： | 江苏省科技厅科技攻关项目 |
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| 摘 要： | 目的]为探索暗纹东方基因组中是否存在BAFF基因。方法]通过比对人、小家鼠、野猪、原鸡的BAFF基因cDNA序列,获取高度保守的同源片断。根据红鳍东方基因组文库序列设计引物,从野生暗纹东方肌肉样品中提取总DNA进行PCR扩增,并进行PCR产物的克隆与测序。结果]通过PCR扩增获得3条特异性条带,大小分别为542、349、221 bp。在红鳍东方基因组文库中未找到与542 bp序列匹配的片段。349和221 bp序列在暗纹东方和红鳍东方中是保守的。暗纹东方与红鳍东方序列比对结果表明,碱基突变的主要方式为插入或缺失。结论]3个扩增条带为BAFF基因相关序列的可能性较小。在鱼类生物体中很可能不存在与哺乳类、鸟类BAFF同源基因。
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| 关 键 词： | 暗纹东方 BAFF基因 分子进化 红鳍东方 |
Discussion on the Properties of the Related Sequence to BAFF Gene in Takifugu obscurus |
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| WEI Cui et al.Discussion on the Properties of the Related Sequence to BAFF Gene in Takifugu obscurus[J].Journal of Anhui Agricultural Sciences,2008,36(10). |
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| Authors: | WEI Cui |
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| Abstract: | Objective] The research aimed to explore whether there was BAFF gene in the genome of Takifugu obscurus.Method] cDNA sequences of BAFF gene from Homo sapiens,Mus musculus,Sus scrofa and Gallus gallus were compared to obtain its highly-conserved homologous fragment.Primers were designed according to the sequences in the genomic library of Takifugu rubripes.Total DNA was extracted from the muscle samples of wild T.obscurus to make PCR amplification and the PCR products were cloned and sequenced.Result] Three specific bands were obtained though PCR amplification,with the size of 542,349,221 bp.The suited fragment with 542 bp sequence was not found in the genomic library of T.rubripes.Sequences with the size of 349 and 221 bp were conservative in T.obscurus and T.rubripes.The comparison results between T.obscurus and T.rubripes showed that the main type of base mutation was insertion or deletion.Conclusion] The possibility that 3 amplified bands were the related sequences to BAFF gene was less.It was probably that the homologous gene with BAFF of mammalian and birds didn't exist in fish organism. |
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| Keywords: | Takifugu obscurus BAFF gene Molecular evolution Takifugu rubripes |
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