Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.) |
| |
| Authors: | Guohao He Channapatna S Prakash |
| |
| Institution: | (1) Center for Plant Biotechnology Research, Tuskegee University, School of Agriculture and Home Economics, Milbank Hall, Tuskegee, AL, 36088, U.S.A |
| |
| Abstract: | The detection of DNA polymorphism in cultivated peanut (Arachis hypogaea L.) is reported here for the first time. The DNA
amplification fingerprinting (DAF) and amplified fragment length polymorphism (AFLP) approaches were tested for their potential
to detect genetic variation in peanut. The AFLP approach was more efficient as 43% of the primer combinations detected polymorphic
DNA markers in contrast to 3% with the DAF approach. However, the number of polymorphic bands identified using primers selected
in both approaches was comparable. In the DAF study, when 559 primers of varying types were screened, 17 (mostly 10-mer types)
detected polymorphism producing an average of 3.7 polymorphic bands per primer with a total of 63 polymorphic markers. In
the AFLP study, when 64 primer combinations (three selective nucleotides) corresponding to restriction enzymes Eco RI and
Mse I were screened, 28 detected polymorphism. On an average, 6.7% of bands obtained from these 28 primer pairs were polymorphic
resulting in a total of 111 AFLP markers. Our results demonstrate that both AFLP and DAF approaches can be employed to generate
DNA markers in peanut and thus have potential in the marker-assisted genetic improvement and germplasm evaluation of this
economically important crop.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
| Keywords: | amplified fragment length polymorphism (AFLP) markers DNA amplification fingerprinting (DAF) groundnut polymerase chain reaction (PCR) random amplified polymorphic DNA (RAPD) markers |
| 本文献已被 SpringerLink 等数据库收录！ |
|
