| 珠芽蓼内生菌Z17抑菌能力测定及其鉴定 |
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| 引用本文： | 李振东,陈秀蓉,杨成德.珠芽蓼内生菌Z17抑菌能力测定及其鉴定[J].草业科学,2011,28(12):2096-2101. |
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| 作者姓名： | 李振东 陈秀蓉 杨成德 |
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| 作者单位： | 1. 甘肃农业大学草业学院草业生态系统教育部重点实验室中一美草地畜牧业可持续发展研究中心,甘肃兰州730070／甘肃省社会科学院农村发展研究所,甘肃兰州730070
2. 甘肃农业大学草业学院草业生态系统教育部重点实验室中一美草地畜牧业可持续发展研究中心,甘肃兰州,730070 |
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| 基金项目： | 国家自然基金项目，甘肃农业大学草业学院草业科学国家级重点学科学术骨干科研项目暨草业生态系统教育部省部共建重点实验室项目 |
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| 摘 要： | 以高寒草地优势植物珠芽蓼(Polygonum viviparum)的一内生细菌菌株Z17为研究对象，采用平板对峙法测定其对7种植物病原真菌的抑菌能力，利用形态学和分子生物学2种方法确定其分类地位。该菌对玉米小斑病菌（Bipolaria maydis）、立枯丝核病菌（Rhizoctonia solani）、菌核病菌（Sclerotinia sclerotiorum）、西瓜尖镰孢(Fusarium oxysporum f.Niveum)、番茄早疫病菌（Alternaria solani）、番茄灰霉病菌（Botrytis cinerea）和小麦离蠕孢菌（Bipolaris sorokiniana）等均有抑制作用，抑菌谱较广。菌体杆状，菌体大小为（1.4～3.6） μm×（0.4～0.6） μm，革兰氏阳性，中生芽孢，与芽孢杆菌的形态一致；16S rDNA基因序列与莫海威芽孢杆菌（Bacillus mojavensis）的模式菌株BCRC 17531相似度达99.79%，在系统发育树上Z17与菌株BCRC 17531的遗传距离小于0.000 5，故鉴定为莫海威芽孢杆菌。该菌可能具有开发生物农药的潜力。
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| 关 键 词： | 高寒草地 内生拮抗菌 莫海威芽孢杆菌 鉴定 |
Identification of Polygonum viviparum endophytic bacteria Z17 and its capacity to antagonistic towards pathogenic fungi |
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| LI Zhen dong,CHEN Xiu rong,YANG Cheng de.Identification of Polygonum viviparum endophytic bacteria Z17 and its capacity to antagonistic towards pathogenic fungi[J].Pratacultural Science,2011,28(12):2096-2101. |
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| Authors: | LI Zhen dong CHEN Xiu rong YANG Cheng de |
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| Abstract: | Polygonum viviparum is the dominant species of alpine meadow community. A experiment was conducted to estimate the capacity of P. viviparum endophytic bacteria Z17 to antagonistic towards pathogenic fungi by PDA plates and to determine the taxonomic status of Z17 by morphological and molecular biology methods.The results of this study showed that Z17 inhibited Bipolaria maydis, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum f. Niveum, Alternaria solani, Botrytis cinerea, Bipolaris sorokiniana, indicating that Z17 had a wide antibacterial spectrum. Z17 was rod shape with a size of （1.4-3.6） μm ×（0.4-0.6） μm, Gram positive, and produced spore in the center, and it was same morphological characteristics with Bacillus sp. Similarity between 16S rDNA gene sequence of Z17 and Bacillus mojavensis model strain BCRC 17531 reached 99.79%, and the genetic distance between Z17 and BCRC 17531 in the phylogenetic tree was less than 0.000 5. These results suggested that Z17 could be identified as Bacillus mojavensis and had the potential to be explored as Biopesticides. |
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| Keywords: | Alpine grassland Antagonistic endophytic bacteria Bacillus mojavensis identification |
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