| 秋子梨等九个品种S基因型的鉴定 |
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| 引用本文： | 张春芳,李茂福,韩振海,龙慎山,李天忠.秋子梨等九个品种S基因型的鉴定[J].北方园艺,2008(11). |
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| 作者姓名： | 张春芳 李茂福 韩振海 龙慎山 李天忠 |
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| 作者单位： | 中国农业大学,园艺植物研究所,北京,100094 |
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| 基金项目： | 国家自然科学基金
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北京市重点实验室资助项目
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| 摘 要： | 根据梨S基因高度保守区设计兼并引物,对各秋子梨品种的基因组进行S基因特异性扩增、连接、转化并测序,GenBank中比较后确定各品种的S基因型.9个供试品种的S基因型分别为:秋子梨中的'寒香'为S36Sx,'苹香'为S1S31,'南果梨'为S34S34,'金香水'为S1Si,'延边大香水'为S31S36,'寒红'为S27S34,'小香水'为S29S34,'花盖王'为S31S31S34S34,白梨中的'锦丰'为S19S34.
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| 关 键 词： | 秋子梨 自交不亲和性 S基因 S基因型鉴定 |
Identification of S-genotypes of Ussurian Pear Cultivars |
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| ZHANG Chun-fang,LI Mao-fu,HAN Zhen-hai,LONG Shen-shan,LI Tian-zhong.Identification of S-genotypes of Ussurian Pear Cultivars[J].Northern Horticulture,2008(11). |
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| Authors: | ZHANG Chun-fang LI Mao-fu HAN Zhen-hai LONG Shen-shan LI Tian-zhong |
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| Institution: | ZHANG Chun-fang,LI Mao-fu,HAN Zhen-hai,LONG Shen-shan,LI Tian-zhong (Institute of Horticultural Plants,China Agricultural University,Beijing 100094,China) |
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| Abstract: | Partial S-RNase genes of Ussurian pear were amplified and cloned by using two pairs of primers based on the conserved regions of pear S-RNase structure in nine cultivars of unknown S-genotypes.The PCR products were sequenced and compared in GenBank.The S-genotypes of the nine tested cultivars were identified as follows:'Hanxiang' was S36Sx,'Pingxiang' was S1S31,'Nanguoli' was S34S34,'Jinxiangshui' was S1Si,'Yanbian Daxiangshui' was S31S36,'Hanhong' was S27S34,'Xiaoxiangshui' was S29S34,'Huagaiwang' was S31S... |
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| Keywords: | Ussurian pear(P ussuriensis Maxim) Self-incompatibility S-RNase genes S-genotyping |
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