| 黄颡鱼微卫星标记筛选及特征分析 |
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| 引用本文： | 刘臻,鲁双庆,张建社,刘红玉,张林达,谢帝芝.黄颡鱼微卫星标记筛选及特征分析[J].农业生物技术学报,2008,16(4). |
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| 作者姓名： | 刘臻 鲁双庆 张建社 刘红玉 张林达 谢帝芝 |
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| 作者单位： | 1. 长沙大学生物工程与环境科学系,长沙,410003
2. 湖南大学环境科学与工程系,长沙,410082 |
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| 基金项目： | 国家自然科学基金，湖南省自然科学基金，湖南省教育厅资助项目 |
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| 摘 要： | 通过磁珠富集法首次筛选黄颡鱼微卫星标记并对其特征进行分析。用生物素标记的寡核苷酸探针(AC)8、(CT)8、(AT)7、(GATA)8、(GATT)7进行微卫星片断的富集，挑选部分阳性克隆测序获得38个含有微卫星的DNA序列，总计设计并合成了22对微卫星引物。以普通黄颡鱼基因组DNA为模板，进行PCR扩增，结果筛选出18个多态性微卫星标记，每对引物等位基因数2-8个（平均3.78），遗传杂合度0.2225-0.8101（平均0.5755），多态信息含量0.3425-0.7900（平均0.5336）。此外18对多态性微卫星引物中有16对在黄颡鱼属其它4种鱼类具有通用性，13对在鲇形目中的3种鱼类具有通用性。因此，可以推断筛选的多态性微卫星标记可用于黄颡鱼属和鲇形目鱼类的遗传分析。
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| 关 键 词： | 黄颡鱼 微卫星标记 筛选及特征 |
| 收稿时间： | 2007-7-12 |
| 修稿时间： | 2007-11-12 |
Isolation and Characterization of Microsatcllitcs in Pseudobagrus fulvidraco |
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| LIU Zhen,LU Shuang-qing,ZHANG Jian-she,LIU Hong-yu,ZHANG Lin-da,XIE Di-zhi.Isolation and Characterization of Microsatcllitcs in Pseudobagrus fulvidraco[J].Journal of Agricultural Biotechnology,2008,16(4). |
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| Authors: | LIU Zhen LU Shuang-qing ZHANG Jian-she LIU Hong-yu ZHANG Lin-da XIE Di-zhi |
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| Abstract: | We report here the isolation and characterization of microsatellites, in Pelteobagrus fulvidraco using an enrichment protocol. The size-fractionated genomic library was screened with (AC)8、(CT)8、(AT)7、(GATA)8 and (GATT)7 oligonucleotide probes. A total of 38 clones were identified as having the microsatellite repeats, and specific 22 primer pairs were designed for 38 microsatellite loci. Of the 22 primer pairs, 18 amplified polymorphic loci in Pelteobagrus fulvidraco . At these polymorphic loci, the number of alleles per locus varied from 2 to 8（the average 3.78）, the observed heterozygosities ranged from 0.2225 to 0.8101（the average 0.5755）, and the polymorphism information content ranged from 0.3425 to 0.7900（the average0.5336）. These 18 primer pairs were examined also in Pelteobagrus species and Siluriformes species.16 of the 18 primer pairs amplified the loci in the Pelteobagrus species,13 of the 18 primer pairs amplified the loci in the Siluriformes species. These results indicate that the microsatellites isolated in this study were highly informative and could be useful tools for genetic analysis in the Pelteobagrus species and the Siluriformes species. |
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