| 荧光定量PCR技术在植物病原检测方面的应用 |
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| 引用本文： | 王义鹏,许艳丽.荧光定量PCR技术在植物病原检测方面的应用[J].大豆通报,2011(2):9-12. |
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| 作者姓名： | 王义鹏 许艳丽 |
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| 作者单位： | 王义鹏,Wang Yipeng(东北林业大学生命科学学院,哈尔滨,150040;中国科学院东北地理与农业生态研究所,哈尔滨,150081);许艳丽,Xu Yanli(中国科学院东北地理与农业生态研究所,哈尔滨,150081) |
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| 基金项目： | 黑龙江省科技计划项目"大豆胞囊线虫病综合防治技术研究"，国家自然科学基金项目"连作大豆胞囊线虫病抑制性土壤特性与发生机制" |
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| 摘 要： | 就实时荧光定量PCR的原理、应用和不足及前景等方面进行了论述。实时荧光定量PCR技术以其快速、灵敏、准确和高通量等特点受到了人们越来越多的重视,被广泛的应用于生物学研究的各个领域。近年来在植物病原检测方面,已建立了一大批植物病原的实时荧光定量PCR方法,这为植物病害的准确诊断与防控提供了强有力的工具。
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| 关 键 词： | 实时荧光定量 植物病害 检测 |
Application of Real-time PCR on detection of plant pathogen |
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| Wang Yipeng,Xu Yanli.Application of Real-time PCR on detection of plant pathogen[J].Soybean Bulletin,2011(2):9-12. |
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| Authors: | Wang Yipeng Xu Yanli |
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| Institution: | Wang Yipeng,Xu Yanli |
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| Abstract: | Real-time PCR principle,application,deficiencies and its prospect were introduced.More and more attentions were paid to Real-time PCR because of its rapidity,sensitivity and high-throughout.The technology was applied in different areas on biological researches.Los of detective methods on detection of plant pathogen using real-time PCR were established and great technical supplies were given to diagnosis,prevention and control of plant diseases. |
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| Keywords: | Real-time PCR Plant pathogen Detection |
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