| 乙二醇及二甲基亚砜对小鼠卵母细胞冷冻后纺锤体及孤雌激活后发育能力的影响 |
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| 引用本文： | 裴燕,索伦,王亮,朱士恩.乙二醇及二甲基亚砜对小鼠卵母细胞冷冻后纺锤体及孤雌激活后发育能力的影响[J].中国畜牧杂志,2010,46(5). |
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| 作者姓名： | 裴燕 索伦 王亮 朱士恩 |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193
2. 中国农业大学动物科技学院,北京,100193;中国农业大学农业生物技术国家重点试验室,北京,100193 |
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| 摘 要： | 试验采用EFS30、DFS30和EDFS30冷冻液对小鼠MⅡ期卵母细胞进行玻璃化冷冻,并对冷冻-解冻后卵母细胞存活率、纺锤体形态正常率、孤雌激活胚胎的发育能力进行研究。结果表明:利用EDFS30冷冻-解冻卵母细胞后的存活率(98.3%)显著高于EFS30组(88.2%)和DFS30组(89.5%)(P0.05);DFS30组纺锤体形态正常率(44.9%)显著低于其他各组;而孤雌激活后EFS30、DFS30、EDFS30和对照组之间卵裂率(59.1%、56.3%、59.3%和60.0%)、囊胚率(52.0%、53.7%、61.2%和62.1%)和囊胚细胞数(49、48、50、50)均无显著性差异(P0.05)。综上所述,3种冷冻液冷冻卵母细胞孤雌激活后均可获得较好的体外发育能力,其中EDFS30较适宜小鼠MⅡ期卵母细胞冷冻保存。
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| 关 键 词： | 乙二醇 二甲基亚砜 玻璃化 纺锤体 小鼠 |
Effect of EG and DMSO on Spindle and Parthenogenetic Development of Matured Oocyte after Vitrification in Mice |
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| PEI Yan,SUO Lun,WANG Liang,ZHU Shi-en.Effect of EG and DMSO on Spindle and Parthenogenetic Development of Matured Oocyte after Vitrification in Mice[J].Chinese Journal of Animal Science,2010,46(5). |
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| Authors: | PEI Yan SUO Lun WANG Liang ZHU Shi-en |
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| Institution: | PEI Yan1,SUO Lun1,WANG Liang1,ZHU Shi-en1,2 (1.College of Animal Science , Technology,China Agricultural University,Beijing 100193,China,2.State Key Laboratory for Agrobiotechnology,China) |
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| Abstract: | The present study was designed to cryopreserve the mouse matured oocytes with vitrification solution EFS30,DFS30 or EDFS30,and then the survival,morphology of spindle after thawing.Moreover,the development potential in vitro after parthenogenetic activation were also detected.The results indicated that the rate of survival of oocytes after thawing in EDFS30 group (98.3%) were significantly higher than that of EFS30 (88.2%) and DFS30 group (89.5%)(P < 0.05);The rate of oocytes with normal spindle in DFS30 gr... |
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| Keywords: | EG DMSO vitrification spindle mice |
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