| 新型鸭呼肠孤病毒病浓缩灭活疫苗的制备及免疫原性分析 |
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| 引用本文： | 陈仕龙,肖世峰,程晓霞,林锋强,王劭,王锦祥,朱小丽,陈少莺.新型鸭呼肠孤病毒病浓缩灭活疫苗的制备及免疫原性分析[J].福建农业学报,2017(11):1189-1192. |
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| 作者姓名： | 陈仕龙 肖世峰 程晓霞 林锋强 王劭 王锦祥 朱小丽 陈少莺 |
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| 基金项目： | 福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1023-9)，福建省农业科学院青年英才项目(YC2016-1) |
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| 摘 要： | 为研制高效的新型鸭呼肠孤病毒病(NDRV)灭活疫苗,分别采用病毒尿囊液和浓缩后病毒尿囊液为抗原,经甲醛灭活处理后与常规油佐剂制成灭活疫苗,并进行种鸭免疫后血清学检测、雏鸭免疫保护及安全试验。结果表明,浓缩疫苗安全性好,能明显提高疫苗的免疫效果,高峰期抗体效价的免疫持续期比常规疫苗长;浓缩疫苗免疫雏鸭后的免疫保护性达100%。以上研究表明,NDRV浓缩疫苗安全、高效,在雏鸭上具有良好的免疫保护效果,具有良好的市场前景。
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Efficacy Evaluation of a Concentrated Inactivated Vaccine against Novel Duckling Reovirus Infection |
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| Abstract: | In this paper,an experimental concentrated inactivated vaccine and a conventional inactivated vaccine against Novel Duckling Reovirus (NDRV) infection were prepared.After one single-dose immunization in adult Cherry Valley ducks,the serum samples collected in a period of 13 weeks were tested for antibodies by serum neutralisation (SN) method.The protective effects against NDRV infection were observed in 3-day-old Cherry Valley ducklings and mule duckling after a single-dose immunization.The serological results showed that concentrated inactivated vaccine was much more immunogenic than the conventional inactivated vaccine,with a much higher SN antibody titers at all test time points.The concentrated inactivated vaccine was highly protective against NDRV challenge at 7-day post-vaccination with a 100 ％ protective efficacy,as compared to 40％-60％ protective efficacy for conventional inactivated vaccine.Taken together,this experimental concentrated inactivated vaccine exhibited immune efficiency much superior to conventional inactivated vaccine and might be a useful vaccine against NDRV infection. |
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