| 白茶实时荧光定量PCR分析中内参基因的筛选与验证 |
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| 引用本文： | 陈静,郑知临,林浥,曹红利,陈笛,陈桂信,叶乃兴.白茶实时荧光定量PCR分析中内参基因的筛选与验证[J].福建农业学报,2017(11):1201-1206. |
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| 作者姓名： | 陈静 郑知临 林浥 曹红利 陈笛 陈桂信 叶乃兴 |
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| 基金项目： | 国家自然科学基金项目(31270735)，福建农林大学科技创新专项(CXZX2016117、CXZX2017313) |
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| 摘 要： | 利用GeNorm,NormFinder,BestKeeper软件对茶树不同叶位及白茶萎凋过程中7个候选内参基因的表达稳定性进行分析。获得3个稳定性较好的内参基因β-Actin、GAPDH和RUBP,RUBP适合作为白茶萎凋过程叶片荧光定量PCR的内参基因,β-Actin适合作为白茶不同叶位叶片荧光定量PCR的最佳内参基因。因此,在白茶萎凋过程中,使用GAPDH+RUBP组合作为内参基因进行荧光定量PCR检测。
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Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea |
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| Abstract: | To select appropriate reference genes to be used in the real-time florescence quantitative PCR analysis for studying the gene expression in of white tea leaves during withering,the software of GeNorm,NormFinder,and BestKeeper were applied.Three stable reference genes,β-Actin,GAPDH and RUBP,were identified.Among them,RUBP was found most adequate for comparing the expressions at various stages of withering,while β-Actin for differentiating them in leaves from different parts of a tea plant.The combination of GAPDH+RUBP was determined as choice reference genes for the analysis. |
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