| 新疆某地牛病毒性腹泻病毒生物型的鉴定 |
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| 引用本文： | 郭燕,王新华,邓宇,陈琛,荣光,夏伦斌,王运宏,钟发刚.新疆某地牛病毒性腹泻病毒生物型的鉴定[J].动物医学进展,2007,28(1):50-52. |
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| 作者姓名： | 郭燕 王新华 邓宇 陈琛 荣光 夏伦斌 王运宏 钟发刚 |
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| 作者单位： | 石河子大学动物科技学院,新疆石河子,832000;新疆农垦科学院畜牧所基因工程实验室,新疆石河子,832000;西昌学院动物科技系,四川西昌,615013 |
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| 摘 要： | 从临床怀疑为牛病毒性腹泻病毒(BVDV)感染奶牛的血液中用RT-PCR方法检测BVDV,目的片段回收克隆测序,经Blast分析,与匈牙利疫苗致弱株同源性99%,并用PCR方法鉴定基因型,均为BVDV-Ⅰ型.用MDBK细胞鉴定生物型,不致细胞病变,为非致细胞病变(nCP)型.
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| 关 键 词： | 牛病毒性腹泻病毒 非致细胞病变 |
| 文章编号： | 1007-5038（2007）01-0050-03 |
| 收稿时间： | 2006-09-30 |
| 修稿时间： | 2006年9月30日 |
Identification of BVDV in Partical Area of Xinjiang |
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| GUO Yan,WANG Xin-hua,DENG Yu,CHEN Chen,RONG Guang,XIA Lun-bin,WANG Yun-hong,ZHONG Fa-gang.Identification of BVDV in Partical Area of Xinjiang[J].Progress In Veterinary Medicine,2007,28(1):50-52. |
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| Authors: | GUO Yan WANG Xin-hua DENG Yu CHEN Chen RONG Guang XIA Lun-bin WANG Yun-hong ZHONG Fa-gang |
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| Abstract: | BVDV were detected from the blood samples of dairy cow infected suspiciously with BVDV by RT-PCR method. The goal fragment was cloned and sequenced. It shared 99% homology with Hungarian vaccine strain. The gene type was identified as BVDV-1 type by PCR. The biological type was identified as non-cell pathogenic type by using MDBK cells. |
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| Keywords: | Bovine viral diarrhoea virus non-cell pathogenicity |
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