| microRNA-152对奶牛乳腺上皮细胞乳蛋白合成的调控作用 |
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| 引用本文： | 王杰,边艳杰,王卓然,李丹,高学军,李庆章,王春梅.microRNA-152对奶牛乳腺上皮细胞乳蛋白合成的调控作用[J].中国畜牧兽医,2014,41(6):6-11. |
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| 作者姓名： | 王杰 边艳杰 王卓然 李丹 高学军 李庆章 王春梅 |
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| 摘 要： |
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Effects of microRNA-152 on Milk Protein Synthesis in Bovine Mammary Epithelial Cells |
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| WANG Jie,BIAN Yan-jie,WANG Zhuo-ran,LI Dan,GAO Xue-jun,LI Qing-zhang,WANG Chun-mei.Effects of microRNA-152 on Milk Protein Synthesis in Bovine Mammary Epithelial Cells[J].China Animal Husbandry & Veterinary Medicine,2014,41(6):6-11. |
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| Authors: | WANG Jie BIAN Yan-jie WANG Zhuo-ran LI Dan GAO Xue-jun LI Qing-zhang WANG Chun-mei |
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| Abstract: | In order to study the effect of microRNA-152 （miR-152） on milk protein synthesis in dairy cow mammary epithelial cells, liposome transfection was used to change the expression of miR-152 in bovine mammary epithelial cells. The influence of miR-152 on the cell proliferation and lactation was detected using Real-time PCR, Western blotting, cell viability analysis and other techniques. Results indicated that when miR-152 was inhibited, SOCS3 protein expression was increased, signaling pathway factors p-STAT5,p-mTOR,S6K1 and cyclinD1 protein expression were decreased, cell proliferation was decreased, β-casein secretion was decreased. The results suggested that miR-152 probably regulated bovine mammary epithelial cells by suppressing expression of SOCS3. And miR-152 could increase milk protein synthesis and cells proliferation by regulating signal transductions of p-STAT5,p-mTOR,S6K1 and cyclinD1 in mammary epithelial cells. |
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