Role of ACSL3 expression in suppressing prostate cancer cell metastasis |
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| Authors: | LI Ke CHEN Yi DONG Yan YE Zhi-qiang |
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| Institution: | 1.Department of Urology, 4Department of Emergency, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;2.Department of Breast Surgery, Sun Yat-sen Memorial Hospital of Sun Yat-sen University,Guangzhou 510120,China;3.Department of Biotechnology, School of Life Science and Technology, Jinan University, Guangzhou 510632, China. |
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| Abstract: | AIM:To analyze the difference of long-chain acyl-CoA synthetase 3 (ACSL3) expression between normal prostate epithelial cells and prostate cancer cells.METHODS:ACLS3 mRNA expression between normal prostate epithelial cells and prostate cancer cells was compared using RT-PCR. Meanwhile, ACSL3 gene was amplified from prostate cancer cells, and the eukaryotic expression plasmid pCDNA3.1(+)-Flag-ACLS3 and lentivirus Lenti-ACLS3 were constructed. After transfection of ACSL3-plasmid and lentivirus into the prostate cancer cells, ACSL3 expressive was detected by RT-PCR and Western blotting, and then Matrigel invasion assay was performed to investigate the alteration of the invasive ability of the prostate cancer cells with over-expression of ACSL3. RESULTS:A significant difference of ACSL3 mRNA level between normal prostate epithelial cells and prostate cancer cells was observed. ACSL3 was highly expressed in localized prostate cancer cells compared to metastatic prostate cancer cells, while ACSL3 expression was higher in androgen-dependent prostate cancer cells than that in androgen-independent prostate cancer cells. Furthermore, the eukaryotic expression plasmid and lentivirus containing ACLS3 gene were successfully constructed. The prostate cancer cell line which stably over-expressed ACLS3 was established. Up-regulation of ACSL3 inhibited the invasive ability of prostate cancer cells. CONCLUSION:ACSL3 plays an antagonistic role in invasiveness of prostate cancer. |
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| Keywords: | Prostate neoplasms Long-chain acyl-CoA synthetase 3 Neoplasm invasiveness |
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