Effects of different antioxidants on MMP-9 protein expression in fetal membrane tissue |
| |
| Authors: | LI Ling CUI Jin-hui CHEN Xin-juan FAN Jian-hui |
| |
| Institution: | Department of Obstetrics, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. |
| |
| Abstract: | AIM：
To find out the influences of different antioxidants on the protein expression of matrix metalloproteinase 9 (MMP-9) in the membrane tissue of premature rupture cultured in vitro. METHODS：Eight pregnant women of premature rupture of membranes (PROM) who had delivered by cesarean section before labor were enrolled in the study and divided into 2 groups: term premature rupture of membranes (TPROM) and preterm premature rupture of membranes (PPROM). The fetal membrane tissues from each case was collected and divided into 3 sub-groups, which were cultured for 24 h with phosphate buffered saline (PBS, control group), 0.5 mmol/L alpha-lipoic acid (α-LA), or 28.8 g/L vitamin C+1.8 g/L vitamin E in vitro. The protein expression of MMP-9 was determined by immunohistochemistry and Western blotting. RESULTS：MMP-9 was located in the chorionic cells and neutrophils in TPROM tissues and there was no significant difference among samples of different treatments. In PPROM tissues, MMP-9 was found not only in the chorionic cells and neutrophils, but also in the amniotic epithelial cells. The integral absorbance values in antioxidant-treated samples were lower than that in PBS-treated samples, and the significant difference between α-LA-treated samples and vitamin C+vitamin E-treated samples was observed. The expression of pro-MMP-9 in TPROM tissues showed no significant difference after treated with different antioxidants, but the active MMP-9 expression was decreased after treated with antioxidants, and the influence of vitamin C+vitamin E was stronger than α-LA. The expression of pro-MMP-9/active MMP-9 in PPROM tissues was decreased after treated with antioxidants, and the influence of vitamin C+vitamin E was stronger than α-LA. CONCLUSION：Antioxidants result in the down-regulation of MMP-9 protein expression, and the influence of vitamin C+vitamin E is stronger than α-LA, indicating that these antioxidants can be used clinically to prevent premature rupture of membranes. |
| |
| Keywords: | Premature rupture of membranes Alpha-lipoic acid Vitamin C Vitamin E Matrix metalloproteinase 9 |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
