| 猪流感病毒H1N1亚型天津分离株核蛋白、基质蛋白、非结构蛋白基因克隆与序列分析 |
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| 引用本文： | 孙英峰,鄢明华,路超,李秀丽,张莉,韩伟,任卫科,田向学.猪流感病毒H1N1亚型天津分离株核蛋白、基质蛋白、非结构蛋白基因克隆与序列分析[J].中国畜牧兽医,2014,41(4):61-66. |
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| 作者姓名： | 孙英峰 鄢明华 路超 李秀丽 张莉 韩伟 任卫科 田向学 |
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| 作者单位： | （天津市畜牧兽医研究所，天津 300381） |
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| 基金项目： | 天津市科技计划（10ZCZDNC02800）；天津市应用基础及前沿技术研究计划（12JCYBJC20100）。 |
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| 摘 要： | 从天津地区不同猪场分离到6株H1N1亚型猪源流感病毒（SIV）。根据GenBank发表的H1N1亚型 SIV的核蛋白（NP）、基质蛋白（M）及非结构蛋白（NS）基因序列，分别设计3对引物，将RT-PCR产物克隆至pMD18-T载体，进行测序分析。遗传进化分析结果表明：A/swine/Tianjin/TJ2/2005（H1N1）与A/swine/Tianjin/TJ4/2006（H1N1）的NP、M及NS基因核苷酸序列在遗传进化树中均与A/swine/Guangdong/33/2006（H1N1）位于同一分支上，属于古典型H1N1猪谱系；A/swine/Tianjin/TJ3/2006（H1N1）与A/swine/Tianjin/TJ8/2006（H1N1)的NP、M及NS基因核苷酸在遗传进化树中均与A/Dunedin/2/2000（H1N1）组成一个大分支，可能起源于人谱系；A/swine/Tianjin/TJ6/2009（H1N1）与A/swine/Tianjin/TJ7/2009（H1N1）NP、M及NS基因核苷酸序列在遗传进化树中均与A/swine/Jiangsu/s15/2011（H1N1）位于同一分支上，属于类禽H1N1猪谱系。本试验对6株H1N1亚型SIV的NP、M及NS全基因序列进行分析，在一定程度上揭示了天津地区H1N1亚型SIV的基因进化与流行情况。
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| 收稿时间： | 2013-11-01 |
Cloning and Sequence Analysis of Nucleoprotein,Matrix Protein and Nonstructural Protein Gene of H1N1 Subtype Swine Influenza Virus Isolated from Tianjin |
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| SUN Ying-feng,YAN Ming-hua,LU Chao,LI Xiu-li,ZHANG Li,HAN Wei,REN Wei-ke,TIAN Xiang-xue.Cloning and Sequence Analysis of Nucleoprotein,Matrix Protein and Nonstructural Protein Gene of H1N1 Subtype Swine Influenza Virus Isolated from Tianjin[J].China Animal Husbandry & Veterinary Medicine,2014,41(4):61-66. |
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| Authors: | SUN Ying-feng YAN Ming-hua LU Chao LI Xiu-li ZHANG Li HAN Wei REN Wei-ke TIAN Xiang-xue |
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| Institution: | （Tianjin Institute of Veterinary and Husbandry Sciences, Tianjin 300381, China） |
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| Abstract: | Six strains of H1N1 subtype swine influenza viruses were isolated from pigs with influenza-like signs in Tianjin. Three pairs of primers were designed based on nucleoprotein (NP), matrix protein (M) and nonstructural protein (NS) genes of the influenza virus H1N1 subtype in GenBank. The NP, M and NS genes were amplified by RT-PCR, which were cloned into pMD18-T vector, sequenced and analyzed. As demonstrated by homology and phylogenic analysis, it was found that the NP, M and NS genes of A/swine/Tianjin/TJ2/2005 (H1N1) and A/swine/Tianjin/TJ4/2006 (H1N1) were higher homologues with A/swine/Guangdong/33/2006, belonging to the classical H1N1 swine lineage. While the A/swine/Tianjin/TJ3/2006 (H1N1) and A/swine/Tianjin/TJ8/2006 (H1N1) were higher homologues with A/Dunedin/2/2000 (H1N1), maybe belonging to human H1N1 lineage. A/swine/Tianjin/TJ6/2009 (H1N1) and A/swine/Tianjin/TJ7/2009 (H1N1) were higher homologues with A/swine/Jiangsu/s15/2011 (H1N1), belonging to the class of avian H1N1 swine lineage. This study showed the evolution and epidemic situation of swine influenza viruses in Tianjin area. |
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| Keywords: | swine influenza H1N1 subtype nucleoprotein gene matrix protein gene nonstructural protein gene sequence analysis |
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