| 布鲁氏菌外膜蛋白22基因克隆与原核表达 |
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| 引用本文： | 杜志强,林涛,刘晓燕,吴亚坤,王建英.布鲁氏菌外膜蛋白22基因克隆与原核表达[J].中国畜牧兽医,2014,41(5):252-254. |
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| 作者姓名： | 杜志强 林涛 刘晓燕 吴亚坤 王建英 |
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| 摘 要： | 本试验以布鲁氏菌外膜蛋白22（omp22）基因作为研究对象，通过基因序列克隆、表达载体构建、大肠杆菌原核表达与亲和纯化，对蛋白的表达与纯化进行了研究。结果显示，omp22基因的核苷酸序列含有639 bp，编码212个氨基酸残基，预测分子质量为22 ku，电泳结果显示重组omp22蛋白的分子质量为47 ku，与理论值相符。本试验结果为进一步研究重组omp22蛋白的免疫刺激与免疫保护作用奠定了基础。
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| 收稿时间： | 2013-11-11 |
Cloning and Prokaryotic Expression of Outer Membrane Protein 22 Gene in Brucella |
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| DU Zhi-qiang,LIN Tao,LIU Xiao-yan,WU Ya-kun,WANG Jian-ying.Cloning and Prokaryotic Expression of Outer Membrane Protein 22 Gene in Brucella[J].China Animal Husbandry & Veterinary Medicine,2014,41(5):252-254. |
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| Authors: | DU Zhi-qiang LIN Tao LIU Xiao-yan WU Ya-kun WANG Jian-ying |
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| Abstract: | In this experiment, the Brucella outer membrane protein 22 （omp22） gene was taken as the research object. Through the gene sequence cloning, expression vector construction, prokaryotic expression and affinity purification, expression and purification of the protein were studied. The results showed that the nucleotide sequence of omp22 gene contained 639 bp, which encoded 212 amino acids residues with predicted molecular weight of 22 ku. The electrophoresis results showed that molecular weight of recombinant omp22 protein was 47 ku, which was consistent with the theoretical value. All these results provided a good basis for immunity stimulation and immunity protective effect of recombinant omp22 protein in further research. |
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