| 牛病毒性腹泻病毒BVDV-JL分离株全基因序列测定与分析 |
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| 引用本文： | 张淑琴,谭斌,王凤雪,程世鹏.牛病毒性腹泻病毒BVDV-JL分离株全基因序列测定与分析[J].中国畜牧兽医,2014,41(10):23-27. |
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| 作者姓名： | 张淑琴 谭斌 王凤雪 程世鹏 |
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| 作者单位： | 中国农业科学院特产研究所, 特种经济动物分子生物学重点实验室, 吉林长春 130112 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费(00320-14016) |
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| 摘 要： | 为了解中国农业科学院特产研究所分子生物学重点实验室前期分离的牛病毒性腹泻病毒BVDV-JL毒株完整的基因序列信息,本试验对BVDV-JL F6代毒株进行了完整基因序列测定。利用反转录—聚合酶链式反应(RT-PCR)方法分段扩增了BVDV-JL分离毒株的7段cDNA片段,分别克隆于pMD18-T载体并进行测序。BVDV-JL株基因组序列全长为12276 bp,编码3901个氨基酸。基因组两端为非编码区5'UTR和3'UTR。基因比对及进化树分析结果表明BVDV-JL与BVDV CP7同源性最高,核苷酸同源性为93.2%,归类于BVDV-1b2基因亚型。BVDV-JL是第1个在中国报道的BVDV-1b2亚型毒株。了解BVDV-JL完整基因序列有利于中国BVDV流行病学调查。
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| 关 键 词： | 牛病毒性腹泻病毒 全基因组 进化树 |
| 收稿时间： | 2014-05-22 |
Complete Genome Sequence Analysis of Bovine Viral Diarrhea Virus BVDV-JL in China |
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| ZHANG Shu-qin,TAN Bin,WANG Feng-xue,CHENG Shi-peng.Complete Genome Sequence Analysis of Bovine Viral Diarrhea Virus BVDV-JL in China[J].China Animal Husbandry & Veterinary Medicine,2014,41(10):23-27. |
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| Authors: | ZHANG Shu-qin TAN Bin WANG Feng-xue CHENG Shi-peng |
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| Institution: | State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant, Chinese Academy of Agricultural Sciences, Changchun 130112, China |
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| Abstract: | The bovine viral diarrhea virus (BVDV) JL strain was isolated from a cattle showing clinical diarrhea signs in Jilin province. In order to analyze the complete genome of BVDV-JL,7 fragments of BVDV-JL were amplified by RT-PCR and cloned into pMD18-T vector then sent for sequencing by commercial serves. Complete genome of the virus was 12276 bp in length and contains a 5'UTR,one open reading frame encoding a polyprotein of 3901 amino acids and a 3'UTR. The results of genomic comparison and phylogenetic analysis showed that BVDV-JL fell into BVDV-1b2 subtype and was most similar to CP7,the nucleotide was 93.2%. BVDV-JL was the first reported BVDV-1b2 strains,BVDV-JL complete genomic sequences enriched the available genomic sequences and it would be benefit to epidemiological surveys of BVDV detected in China. |
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| Keywords: | bovine viral diarrhea virus complete genome phylogeny |
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