| Abstract: | Relative expression of keratin associated protein 8-1 （KAP8-1）gene was determined in different vrieties of Ovis aries skin and hair follicle so as to identify the regulation of KAP8-1 gene on skin and hair follicle development and wool quality difference. Using Real-time PCR SYBR GreenⅠ, Ct of expression of KAP8-1 gene was determined and relative expression of KAP8-1 gene was analyzed by 2-△△Ct. The results showed that there was KAP8-1 gene expression in there different crossbred varieties of Ovis aries (South African mutton Merino sheep（♂）× Northeast Fine wool sheep（♀），South African mutton Merino sheep（♂）× Small-tailed Han sheep（♀），Dorper sheep（♂）× Small-tailed Han sheep（♀）) skin and hair follicle. The ratio of relative expression was 7.56∶2.19∶1. In a word, KAP8-1 gene played a role in regulating skin and hair follicle development and wool quality difference,with difference among different varieties. |
