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| AM 真菌和PGPR 菌剂组合对低温胁迫下黄瓜生长及防御酶活性的影响 | |
| 引用本文： | 韩亚楠,刘润进,李敏.AM 真菌和PGPR 菌剂组合对低温胁迫下黄瓜生长及防御酶活性的影响[J].中国蔬菜,2014,1(7):35. |
| 作者姓名： | 韩亚楠 刘润进 李敏 |
| 作者单位： | （;1. 青岛农业大学菌根生物技术研究所，山东青岛 266109；;2. 青岛农业大学园艺学院，山东青岛 266109） |
| 基金项目： | “十二五”国家科技支撑计划重点项目（2014BAD05B03）， 山东省科技发展计划项目（2013GNC11014），青岛市基础研究项目 （13-1-4-143-jch） |
| 摘 要： | 为了筛选适于黄瓜耐低温的最佳丛枝菌根（AM）真菌和PGPR 组合菌剂，研究了不同AM 真菌、PGPR 组合菌剂对
低温胁迫下黄瓜生长及防御酶活性的影响。结果表明低温胁迫下，单接种AM 真菌或是单接种PGPR 的处理，其黄瓜生长量
及SOD、POD 和CAT 活性均高于不接种对照，而MDA 含量则显著低于不接种对照。AM 真菌接种处理中以接种地表球囊霉
（Glomus versiforme）处理的黄瓜生长量较大、防御酶活性较高、细胞膜受伤害较小；PGPR 接种处理中以接种多粘类芽孢
杆菌（Paenibacillus polymyxa）的处理黄瓜生长量较大、防御酶活性较高、细胞膜伤害较小。AM 真菌和PGPR 双接种处理中，
以地表球囊霉+ 多粘类芽孢杆菌处理生长量最大、防御酶活性最高、细胞膜伤害最轻。可以考虑在今后温室黄瓜种植中添加
该组合菌剂促进黄瓜生长，提高植株耐冷性。 |
| 关 键 词： | 低温胁迫 AM 真菌 PGPR 黄瓜 防御酶 丙二醛 |
Effects of Arbuscular Mycorrhizal Fungi and PGPR Combination Agents on Growth and Defense Enzyme Activity of Cucumber under Low Temperature Stress |
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| HAN Ya-Nan,LIU Run-Jin,LI Min.Effects of Arbuscular Mycorrhizal Fungi and PGPR Combination Agents on Growth and Defense Enzyme Activity of Cucumber under Low Temperature Stress[J].China Vegetables,2014,1(7):35. | |
| Authors: | HAN Ya-Nan LIU Run-Jin LI Min |
| Institution: | （;1.Mycorrhizal Biotechnology Institue of Qingdao Agricultural University，Qingdao 266109，Shandong，China；;2.Horticulture College of Qingdao Agricultural University，Qingdao 266109，Shandong，China） |
| Abstract: | In order to select the best combination of arbuscular mycorrhizal（AM） fungi and plant growthpromoting rhizobacteria（PGPR） for cucumber（ Cucumis sativus）to resist low-temperature damages，we studied the effects of different combinations on growth and defense enzymes in greenhouse under low temperature stress conditions. The results showed that the growth of cucumber and SOD，POD，CAT activities were higher than that of the non-vaccinated control，while MDA content was significantly lower than that of the controls， when inoculation with AM fungi or PGPR under low temperature treatments. Between the AM fungi inoculation treatments，Glomus versiforme had better positive effect on growth，defensive enzyme activities，and decreased the cell membrane damage. Between the PGPR inoculation treatments，Paenibacillus polymyxa had better growth and higher defensive enzyme activities，with less cell membrane damage. Among the inoculation treatments with AM fungi and PGPR ，inoculation treatment with both Glomus versiforme and Paenibacillas polymyxa had the best growth，highest defensive enzyme activities，minimum cell membrane damage. Hence，we would suggest to add this combination in greenhouse cucumber production to promote cucumber growth and improve plant cold resistance. |
| Keywords: | Low temperature stress AM fungi PGPR Cucumber Defense enzymes MDA |
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