| 水牛信号转导子和转录激活子1基因电子克隆及序列分析 |
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| 引用本文： | 邓廷贤,庞春英,陈明棠,王建,杨炳壮,梁贤威.水牛信号转导子和转录激活子1基因电子克隆及序列分析[J].中国畜牧兽医,2014,41(8):9-13. |
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| 作者姓名： | 邓廷贤 庞春英 陈明棠 王建 杨炳壮 梁贤威 |
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| 作者单位： | 中国农业科学院广西水牛研究所，农业部(广西)水牛遗传繁育重点实验室，广西南宁 530001 |
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| 基金项目： | 桂渔牧科（1204917）；桂渔牧科（1304512）；广西‘特聘专家’专项；国家国际科技合作专项（2014DFA31970）。 |
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| 收稿时间： | 2014-02-14 |
Silico Cloning and Sequence Analysis of STAT1 Gene in Water Buffalo (Bubalus bubalis) |
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| DENG Ting-xian,PANG Chun-ying,CHEN Ming-tang,WANG Jian,YANG Bing-zhuang,LIANG Xian-wei.Silico Cloning and Sequence Analysis of STAT1 Gene in Water Buffalo (Bubalus bubalis)[J].China Animal Husbandry & Veterinary Medicine,2014,41(8):9-13. |
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| Authors: | DENG Ting-xian PANG Chun-ying CHEN Ming-tang WANG Jian YANG Bing-zhuang LIANG Xian-wei |
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| Institution: | Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture (Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China |
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| Abstract: | The buffalo STAT1 gene was silico cloned and analyzed by bioinformatics method in this study. Using the Bos taurus STAT1 sequences to get and identify the buffalo STAT1 gene sequences by the silico cloning and RT-PCR technology. The results showed that the buffalo STAT1 gene length size was 3437 bp, contained an open reading frame (ORF) of 2244 bp that encoded 747 amino acids. The CDS of the obtained buffalo STAT1 gene showed that 99%，98%，89%，93%，86%，85% and 87% identity with that of Bos taurus, Ovi saries, Homo sapiens, Sus scrofa, Rattus norvegicus, Mus musculus and Capra hircus. Analysis of the phylogenetic tree showed that the nearest relationship existed between buffalo and Bos taurus. |
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