| 尼龙袋法和改良体外三步法评定瘤胃保护性赖氨酸的瘤胃降解率及小肠消化率 |
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| 引用本文： | 刘保仓,陈傲东,张博文,孔平,刘晨黎,高巍.尼龙袋法和改良体外三步法评定瘤胃保护性赖氨酸的瘤胃降解率及小肠消化率[J].中国畜牧兽医,2014,41(7):108-111. |
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| 作者姓名： | 刘保仓 陈傲东 张博文 孔平 刘晨黎 高巍 |
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| 摘 要： | 本试验旨在利用尼龙袋法和改良体外三步法评定脂肪粉包被赖氨酸（Lys）的瘤胃降解率及小肠消化率。选用3只安装有永久性瘤胃瘘管的体重为（28.0±2.97）kg的5月龄哈萨克公羊，代谢笼内单笼饲养，每天饲喂棉籽壳200 g、精料500 g、小麦秸400～500 g，余料不超过10%。结果表明，该脂肪粉包被赖氨酸的干物质（DM）及粗蛋白质（CP）在瘤胃内的有效降解率均为20%，瘤胃保护率达到80%。经胃蛋白酶和胰酶培养24 h后，DM及CP的消化率分别达到63.6%和98.8%。综上所述，该脂肪粉包被赖氨酸可作为一种新型有效的反刍动物氨基酸补充形式。
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| 关 键 词： | 瘤胃保护性赖氨酸 有效降解率 小肠消化率 瘤胃 绵羊 |
| 收稿时间： | 2013-11-29 |
Determination of Rumen Degradability and Intestinal Digestibility of Ruminally Protected Lysine by Nolon Bag Technique and Modified in vitro Three-step Procedure |
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| LIU Bao-cang,CHEN Ao-dong,ZHANG Bo-wen,KONG Ping,LIU Chen-li,GAO Wei.Determination of Rumen Degradability and Intestinal Digestibility of Ruminally Protected Lysine by Nolon Bag Technique and Modified in vitro Three-step Procedure[J].China Animal Husbandry & Veterinary Medicine,2014,41(7):108-111. |
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| Authors: | LIU Bao-cang CHEN Ao-dong ZHANG Bo-wen KONG Ping LIU Chen-li GAO Wei |
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| Institution: | College of Animal Science & Technology，Shihezi University，Shihezi 832000，China |
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| Abstract: | This trial was conducted to evaluate the rumen degradability and intestinal digestibility of the fat dry powder coated lysine by nylon bag technique and standard three-step in vitro procedure.Three and 5 months old rumen cannulated Kazakh wethers weighed in average （28.0±2.97）kg body weight were housed in metabolic crate singly.The daily diet was composed of cottonseed hull 200 g，concentrate 500 g，wheat straw 400 to 500 g，and the daily refusals were less than 10%.The results showed that the effective degradability of DM and CP of fat dry powder coated lysine in the rumen were all about 20%，the rumen bypass rate achieved 80% or more.After incubation in the pepsin solution and pancreatin solution for 24 h，the in vitro intestinal digestibility of DM and CP were 63.6% and 98.8%，respectively.In summary，This fat dry powder coated lysine could be used as a novel and effective amino acid supplement in the form of ruminant animals. |
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