| 华南地区肉鸽源沙门氏菌的鉴定及遗传进化分析 |
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| 引用本文： | 李雪雁,付涛,纪桂云,张津津,江奕容,林晓霜.华南地区肉鸽源沙门氏菌的鉴定及遗传进化分析[J].中国畜牧兽医,2014,41(7):207-211. |
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| 作者姓名： | 李雪雁 付涛 纪桂云 张津津 江奕容 林晓霜 |
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| 摘 要： | 本试验从华南地区发病肉鸽肠道和肝脏中用BS培养基分离、纯化沙门氏菌，并进行多项生化试验和血清型鉴定试验，结果显示各项生化检测指标均符合沙门氏菌的特点；血清型鉴定试验结果表明该分离菌属沙门氏菌属A-F群。进一步对该分离菌的16S rRNA片段测序及遗传进化分析，确定该分离菌为沙门氏菌菌株，与鼠伤寒沙门氏菌同源性为100%。对该分离菌进行动物回归试验，结果表明该沙门氏菌菌株为华南地区肉鸽的致病菌株。该结果为进一步研究华南地区肉鸽沙门氏菌的致病机理和防控奠定了基础。
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| 关 键 词： | style='font-family: 宋体 font-size: 10.5pt mso-bidi-font-family: 'Times New Roman' mso-bidi-font-size: 12.0pt mso-font-kerning: 1.0pt mso-ansi-language: EN-US mso-fareast-language: ZH-CN mso-bidi-language: AR-SA' lang='EN-US'>pigeon style='mso-bidi-font-style: normal'>Salmonella isolation and identification 16S rRNA genetic evolution analysis |
| 收稿时间： | 2014-01-20 |
Identification and Genetic Evolution Analysis of Salmonella Isolated from Pigeon in South China |
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| LI Xue-yan,FU Tao,JI Gui-yun,ZHANG Jin-jin,JIANG Yi-rong,LIN Xiao-shuang.Identification and Genetic Evolution Analysis of Salmonella Isolated from Pigeon in South China[J].China Animal Husbandry & Veterinary Medicine,2014,41(7):207-211. |
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| Authors: | LI Xue-yan FU Tao JI Gui-yun ZHANG Jin-jin JIANG Yi-rong LIN Xiao-shuang |
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| Abstract: | Salmonella was isolated using BS selective medium from liver and intestinal contents of diseased pigeon raising in South China and made the purified culture, biochemical test, serotype experiment, sequencing analysis of 16S rRNA and regression test. A single colony of Salmonella was isolated, identified by biochemical experiments. Serotype experiment result combined with the biochemical testing results were showed that the strain was the result of A polyvalent F. After aligning the sequence of 16S rRNA fragments with the sequences in GenBank, the results of genetic evolution analysis showed that the similarity of pigeon Salmonella and Salmonella paratyphi was 100%.In the animal regression test,it showed that the Salmonella isolated in pigeon was pathopoiesis. |
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