| 牦牛Toll样受体5基因的克隆及序列分析 |
| |
| 引用本文： | 陈亚冰,兰道亮,林宝山,黄偲,黄勇,李键.牦牛Toll样受体5基因的克隆及序列分析[J].中国畜牧兽医,2014,41(7):30-34. |
| |
| 作者姓名： | 陈亚冰 兰道亮 林宝山 黄偲 黄勇 李键 |
| |
| 摘 要： | 根据GenBank中已公布牛Toll样受体5（toll-like receptors 5，TLR5）基因序列，设计全长引物，并对所得序列进行生物信息学分析和预测。基因序列分析结果表明，克隆得到牦牛TLR5基因全长2582 bp，开放阅读框2577 bp，编码氨基酸858个，N端含有21个氨基酸组成的信号肽，分子质量为97.981 ku,理论等电点为4.85；蛋白预测结果表明，TLR5编码蛋白整体表现为亲水性；同源性分析结果表明，牦牛与野牦牛、黄牛、绵羊、山羊等物种具有较高的同源性，在系统发育树中距离最近，说明TLR5基因序列具有较高的保守性。牦牛TLR5基因序列的成功克隆为今后深入研究牦牛及高原动物抗病及免疫机制提供非常重要的理论基础。
|
| 收稿时间： | 2014-01-20 |
Cloning and Sequence Analysis of TLR5 Gene from Yak |
| |
| CHEN Ya-bing,LAN Dao-liang,LIN Bao-shan,HUANG Cai,HUANG Yong,LI Jian.Cloning and Sequence Analysis of TLR5 Gene from Yak[J].China Animal Husbandry & Veterinary Medicine,2014,41(7):30-34. |
| |
| Authors: | CHEN Ya-bing LAN Dao-liang LIN Bao-shan HUANG Cai HUANG Yong LI Jian |
| |
| Abstract: | This study aimed to investigate the characteristics of the TLR5 gene from yak. According to the published cattle TLR5 gene sequence in the GenBank, we designed special primers for the TLR5 gene. The results of sequence analysis showed that the length of the cloning TLR5 cDNA was 2582 bp，contained a complete 2577 bp open reading frame(ORF). It encoded a peptide of 858 amino acids whose N-terminal signal peptide contained 21 amino acids, and the protein had a calculated molecular weight of 97.981 ku and a PI of 4.85. The results of protein prediction showed that TLR5 protein perform as the hydrophilic. Sequence homology analysis showed that yak had higher homology with wild yak, cattle, sheep and goat. The results showed that TLR5 gene sequence was conserved in mammals. The successful cloning of TLR5 gene of yak provided important basis for the study of molecular mechanism of innate immune response. |
| |
| Keywords: | |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
