| 猪流感病毒LAMP检测方法的建立 |
| |
| 引用本文： | 张莉,鄢明华,李秀丽,路超,王利丽,韩伟,孙英峰.猪流感病毒LAMP检测方法的建立[J].中国畜牧兽医,2014,41(7):64-68. |
| |
| 作者姓名： | 张莉 鄢明华 李秀丽 路超 王利丽 韩伟 孙英峰 |
| |
| 摘 要： | 本试验针对猪流感病毒（swine influenza virus，SIV）NP基因保守区域设计并合成6条引物，建立了SIV的环介导等温扩增（LAMP）检测方法，并进行了特异性、敏感性和重复性试验。结果显示，该方法可特异性检测H1N1、H1N2、H3N2、类禽H1N1亚型SIV及甲型H1N1流感病毒，但不能检测猪繁殖与呼吸综合征病毒、猪细小病毒、猪瘟病毒、猪伪狂犬病病毒、猪圆环病毒2型、日本乙型脑炎病毒；该方法的最低检测量为100拷贝/μL质粒DNA。结果表明建立的LAMP方法具有较高的敏感性和特异性，可用于SIV的快速检测。
|
| 关 键 词： | 猪流感病毒 环介导等温扩增 检测 |
| 收稿时间： | 2014-01-02 |
Establishment of Loop-mediated Isothermal Amplification for Detection of Swine Influenza Virus |
| |
| ZHANG Li,YAN Ming-hua,LI Xiu-li,LU Chao,WANG Li-li,HAN Wei,SUN Ying-feng.Establishment of Loop-mediated Isothermal Amplification for Detection of Swine Influenza Virus[J].China Animal Husbandry & Veterinary Medicine,2014,41(7):64-68. |
| |
| Authors: | ZHANG Li YAN Ming-hua LI Xiu-li LU Chao WANG Li-li HAN Wei SUN Ying-feng |
| |
| Abstract: | The objective of this study was to establish a loop-mediated isothermal amplification(LAMP) method for swine influenza virus （SIV）. Six primers were designed according to the SIV NP gene sequence. Then specificity, sensitivity and reproducibility of the test were carried out. In the results, the developed system was specific for the detection of H1N1, H1N2, H3N2, avian-like H1N1 subtype SIV and H1N1 subtype influenza A, there was no cross-reactivity with porcine reproductive and respiratory syndrome virus, porcine parvovirus, classical swine fever virus, pseudorabies virus, porcine circovirus type 2 and Japanese encephalitis virus. The sensitivity of this assay was 100 copies/μL plasmid DNA. These results showed that LAMP method had higher sensitivity and specificity, and could be used for the detection of SIV. |
| |
| Keywords: | |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
