Detection of the Main 8 Virulence Genes of Staphylococcus aureus from Bovine Mastitis |
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| Authors: | XIE Xiu-lan LIU Xi-yuan WANG Jian-dong FAN Chun-mei YANG Hui-jun KANG Xiao-dong |
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| Institution: | 1. Research Center of Grass and Livestock, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China;2. School of Agriculture, Ningxia University, Yinchuan 750021, China;3. Jinfeng Bureau of Agriculture, Animal Husbandry and Water of Yinchuan, Yinchuan 750002, China |
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| Abstract: | The aim of the present study was to investigate the main virulence genes properties of Staphylococcus aureus (SA) strains from bovine mastitis.For this purpose,8 kinds of virulence genes were detected from 70 isolates of clinical mastitis and 55 isolates of subclinical mastitis with PCR.The results were as follows,the detecting rates of nuc,ClfA,TSST-1,PVL,Hla,Hlb,FnBPA and FnBPB were 100.0% (70 isolates),100.0% (70 isolates),0 (0 isolate),5.7% (4 isolates),100.0% (70 isolates),11.4% (8 isolates),97.1% (68 isolates) and 100.0% (70 isolates) respectively in clinical mastitis;whereas 100.0% (55 isolates),100.0% (55 isolates),0 (0 isolate),70.9% (39 isolates),98.2% (54 isolates),9.1% (5 isolates),100.0% (55 isolates) and 100.0% (55 isolates) in subclinical mastitis. The results implied that nuc,ClfA,Hla,Hlb and FnBPA were the main virulence genes in SA isolates both in clinical mastitis and subclinical mastitis. PVL gene might be a key pathogenic gene for the cows with subclinical mastitis. |
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| Keywords: | bovine mastitis Staphylococcus aureus (SA) virulence genes PCR |
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