| 抗氧氟沙星单克隆抗体的制备及ELISA快速试剂盒的初步研制 |
| |
| 引用本文： | 董李学,冯才伟,冯静,郑百芹,贾芳芳,蒙君丽,郝立武,张书宏.抗氧氟沙星单克隆抗体的制备及ELISA快速试剂盒的初步研制[J].中国畜牧兽医,2014,41(8):90-94. |
| |
| 作者姓名： | 董李学 冯才伟 冯静 郑百芹 贾芳芳 蒙君丽 郝立武 张书宏 |
| |
| 作者单位： | 1.唐山市畜牧水产品质量监测中心，河北唐山 063000；2.北京勤邦生物技术有限公司，北京 102206 |
| |
| 基金项目： | 十二五“国家科技支撑计划项目《食品质量安全控制及检测关键技术与产品》之课题”水产品加工过程质量安全控制关键技术集成与示范（2012BAD29B06）。 |
| |
| 摘 要： | 本试验通过碳二亚胺法，将半抗原氧氟沙星与载体牛血清白蛋白（BSA）和卵清白蛋白（OVA）偶联，制备得到氧氟沙星免疫原和包被原，并制备抗氧氟沙星的单克隆抗体，初步研制测定氧氟沙星的ELISA试剂盒，经过测试，对鱼肉样本中氧氟沙星的检测限为1 μg/kg，添加回收率在62.8%～97.7%之间，批内、批间试验的相对标准偏差均小于10%。
|
| 收稿时间： | 2014-02-07 |
Preparation of Monoclonal Antibodies against Ofloxacin and Preliminary Development of its Enzyme Linked Immunosorbent Assay Kit for Rapid Detection |
| |
| DONG Li-xue,FENG Cai-wei,FENG Jing,ZHENG Bai-qin,JIA Fang-fang,MENG Jun-li,HAO Li-wu,ZHANG Shu-hong.Preparation of Monoclonal Antibodies against Ofloxacin and Preliminary Development of its Enzyme Linked Immunosorbent Assay Kit for Rapid Detection[J].China Animal Husbandry & Veterinary Medicine,2014,41(8):90-94. |
| |
| Authors: | DONG Li-xue FENG Cai-wei FENG Jing ZHENG Bai-qin JIA Fang-fang MENG Jun-li HAO Li-wu ZHANG Shu-hong |
| |
| Institution: | 1.Tangshan Livestock and Aquatic Products Quality Monitoring Center, Tangshan 063000, China；2.Beijing Kwinbon Biotechnology Company, Beijing 102206, China |
| |
| Abstract: | Ofloxacin was coupled with BSA as an immunogen and with OVA as a coating antigen using the coupling agent EDC. Monoclonal antibodies against ofloxacin and its enzyme linked immunosorbent assay kit were prepared and established,respectively. The limit of detection was 1 μg/kg in fish, and the recovery range was 62.8% to 97.7%. The intra and inter assay RSD were both less than 10%. |
| |
| Keywords: | ofloxacin monoclonal antibodies enzyme linked immunosorbent assay kit rapid detection |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
