Effects of hydrogen sulfide on high glucose-induced injury of mouse glomerular podocytes |
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| Authors: | LIU Ye PENG Li LU Sheng-xia DU Yue-juan LIU Yuan-tao FU Yu-qin |
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| Institution: | 1.Department of Nephrology, 2Department of Endocrinology, The Second Hospital of Shandong University, Jinan 250033, China;3.Department of Medicine, Shandong Electric Power Central Hospital, Jinan 250002, China;4.Department of Nephrology, Central Hospital of Jinan City, Jinan 250013, China. |
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| Abstract: | AIM: To investigate the effect of hydrogen sulfide (H2S) on high glucose （HG）-induced injury of the mouse podocyte cell line MPC5. METHODS: The cultured MPC5 cells were randomly divided into 4 groups: HG group, normal glucose (NG) group, NG+DL-propargylglycine (PPG) group, and HG+NaHS group. After treated for a certain time, the cells were collected for further detection. The expression of zonula occludens-2 (ZO-2), nephrin, β-catenin and cystathionine γ-lyase (CSE) was determined by Western blotting. RESULTS: High glucose significantly reduced the expression of nephrin, ZO-2 and CSE (P<0.05), while the level of β-catenin was elevated obviously (P<0.05), all in a time-dependent manner. NG+PPG inhibited the levels of ZO-2 and nephrin significantly (P<0.05), and increased the level of β-catenin (P<0.05), all in a PPG concentration-dependent manner. HG+NaHS induced a more significant increase in the levels of ZO-2 and nephrin as compared with HG group (P<0.01), whereas a severe reduction of β-catenin in HG+NaHS group was observed as compared with HG group. Compared with NG group, the expression of ZO-2 and nephrin was decreased obviously, and the level of β-catenin was increased in HG+NaHS group. CONCLUSION: Down-regulation of CSE contributes to hyperglycemia-induced podocyte injury. Exogenous H2S protects against hyperglycemia-induced podocyte injury, possibly through up-regulation of ZO-2 and subsequent suppression of Wnt/β-catenin pathway. |
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| Keywords: | Diabetic nephropathy Hydrogen sulfide Podocytes Cystathionine γ-lyase Zonula occludens-2 protein β-catenin |
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