| 兴义鸭肌肉生长抑制素基因多态性与屠宰性状的相关性分析 |
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| 引用本文： | 彭邦星,李辉,易恒洁,赵忠海.兴义鸭肌肉生长抑制素基因多态性与屠宰性状的相关性分析[J].中国畜牧兽医,2014,41(12):225-230. |
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| 作者姓名： | 彭邦星 李辉 易恒洁 赵忠海 |
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| 作者单位： | 1. 贵州大学高原山地动物遗传育种与繁殖教育部重点实验室, 贵州贵阳 550025;2. 贵州大学动物科学学院, 贵州贵阳 550025;3. 贵州省动物遗传育种与繁殖重点实验室, 贵州贵阳 550025 |
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| 基金项目： | 教育部科学技术研究重点项目(211168). |
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| 摘 要： | 为了解兴义鸭肌肉生长抑制素(myostatin,MSTN)基因SNPs与屠宰性状的相关性,本研究采用基因克隆及PCR产物直接测序的方法,将MSTN基因作为鸭屠宰性状的候选基因,对兴义鸭的MSTN基因外显子进行多态性检测.结果表明,在60个兴义鸭个体中筛选出8个SNPs,其中,第1外显子有5个突变位点:SNP1(G77A)、SNP2(A91G)、SNP3(G130A)、SNP4(C325T)和SNP5(C331T);在第2外显子中并未发现突变位点;第3外显子有3个突变位点:SNP6(C206T)、SNP7(A235G)和SNP8(C256A);对这8个SNPs与屠宰性状进行关联性分析,结果并未达到显著水平(P>0.05).本研究结果可丰富MSTN基因的研究数据,为鸭的育种提供参考.
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| 关 键 词： | 肌肉生长抑制素基因 兴义鸭 屠宰性状 |
| 收稿时间： | 2014-05-26 |
Polymorphisms of MSTN Gene and its Relationship with Slaughter Traits in Xingyi Duck |
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| PENG Bang-xing,LI Hui,YI Heng-jie,ZHAO Zhong-hai.Polymorphisms of MSTN Gene and its Relationship with Slaughter Traits in Xingyi Duck[J].China Animal Husbandry & Veterinary Medicine,2014,41(12):225-230. |
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| Authors: | PENG Bang-xing LI Hui YI Heng-jie ZHAO Zhong-hai |
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| Institution: | 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;2. College of Animal Science, Guizhou University, Guiyang 550025, China;3. Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, Guiyang 550025, China |
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| Abstract: | In order to understand the relationship between single nucleotide polymorphism sites(SNPs)of myostatin(MSTN) gene and slaughter traits in Xingyi duck, MSTN gene was used as a candidate gene for slaughter traits. The polymorphisms of the exon of MSTN gene in Xingyi duck were detected by direct sequencing of PCR products and gene cloning. The results showed that there were 8 single nucleotide polymorphism sites (SNPs) in 60 Xingyi ducks, there were 5 mutations in exon 1 of SNP1(G77A), SNP2(A91G), SNP3(G130A), SNP4 (C325T), SNP5(C331T), there was no mutation in exon 2, and there were 3 mutations in exon 3 of SNP6(C206T), SNP7 (A235G), SNP8 (C256A). By correlation analysis between the 8 SNPs and slaughter traits, it showed that there were no significant associations between the genetype and slaughter traits of Xingyi ducks (P>0.05). The result of this study could enrich MSTN gene research data and provide reference for duck breeding. |
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| Keywords: | MSTN gene Xingyi duck slaughter traits |
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